fcεri, the high affinity receptor for ige plays a critical role in triggering the allergic reactions. it is responsible for inducing mast cell degranulation and deliberation of allergy mediators when it is aggregated by allergen and ige complexes. fcεri on the mast cells consists of three subunits; α chain directly binds ige, β chain and dimmer of γ chains together mediate intracellular signali...

Despite the great therapeutics potential, sustained release of small interfering RNA (siRNA) remains a challenge. Development siRNA nanoparticle provides possibility to further enhance therapeutic efficacy in gene-based therapy. Herein, we present new system based on encapsulation siRNA/chitosan-methacrylate (CMA) complexes into liposomes form UV crosslinkable Nanolipogels (NLGs) with siRNA-rel...

objective(s): this study was designed to investigate the effect of receptor for advanced glycation end products (rage), s100a12 and c-reactive protein (crp) on the release of circulating endothelial cells (cecs) from human coronary artery endothelial cells (hcaecs). materials and methods: hcaecs were cultured in increasing concentration of crp (0, 12.5, 25, 50μg/ml) or s100a12 protein (0, 4, 10...

background: autophagy suppression recently has been known to have a remarkable effect for cellular adjustment and viability in the final stages of cancer. on the other hand, autophagy has the potential effect in preventing many viruses from replication. beclin1 is the most substantial constituent in autophagy apparatus regulation. this study was intended to investigate the beclin1 sirna knockdo...

background: electroporation is a valuable tool for small interfering rna (sirna) delivery into cells because it efficiently transforms a wide variety of cell types. since electroporation condition for each cell type must be determined experimentally, this study presents an optimal electroporation strategy to reproducibly and efficiently transfect mda-mb 468 human breast cancer cell with sirna. ...

Objective(s): This study was designed to investigate the effect of receptor for advanced glycation end products (RAGE), S100A12 and C-reactive protein (CRP) on the release of circulating endothelial cells (CECs) from human coronary artery endothelial cells (HCAECs). Materials and Methods: HCAECs were cultured in increasing concentration of CRP (0, 12.5, 25, 50μg/ml) or S100A12 protein (0, 4, 1...

Background: Introduction of the RNA interference (RNAi) machinery has guided the researchers to discover the function of essential vital or virulence factor genes in the microorganisms such as fungi. In the filamentous fungus Aspergillus nidulans, the gene sidB plays an essential role in septation, conidiation and vegetative hyphal growth. In the present study, we benefited from the RNAi strate...