In this study, we investigated the ability of curcumin alone or in combination with GLUT1 siRNA to radiosensitize laryngeal carcinoma (LC) through induction autophagy. Protein levels tumour tissues and LC cells were measured by immunohistochemistry Western blotting. vitro, cell proliferation, colony formation assays, death autophagy detected. A nude mouse xenograft model was established injecti...

In this paper, we describe denaturing and non-denaturing ion-pair reversed-phase liquid chromatography (RPLC) methods for analyzing small interfering ribonucleic acid (siRNA). Drug formulations consisting of one or two siRNA duplexes were analyzed in conditions at a low column temperature to separate intact from single-stranded oligonucleotide contaminants quantify them. method, used an elevate...

In recent years, gene therapy has made tremendous progress in the development of disease treatment. Among them, siRNA offers specificity silencing, ease synthesis, and short period, been intensively studied worldwide. However, as hydrophilic polyanion is easily degraded vivo poorly taken up into cells so, benefits its powerful silencing ability will not be realized until better carriers are dev...

Idiopathic pulmonary fibrosis (IPF) is the most common and deadly form of interstitial lung disease. Osteopontin (OPN), a matricellular protein with proinflammatory profibrotic properties, plays major role in several fibrotic diseases, including IPF; OPN highly upregulated patients’ samples. In this study, we knocked down bleomycin (BLM)-induced (PF) mouse model using small interfering RNA (siR...

Multiple gene silencing is being required to target and tangle metabolic pathways in eukaryotes and researchers have to develop a subtle method for construction of RNA interference (RNAi) cassettes. Although, several vectors have been developed due to different screening and cloning strategies but still some potential limitations remain to be dissolved. Here, we worked out a simple cloning stra...

The formation of electrostatic interactions between polyanionic siRNA and polycations gives an easy access to the colloidal particles capable delivering in vitro or vivo. Among used for delivery, chitosan occupies a special place due its unique physicochemical biological properties. In this chapter we describe fundamental practical aspects complexes siRNA. basis complexation oppositely charged ...