efg1 transcription factor is believed to be the main regulator of hyphal formation under many different conditions. in addition, it is responsible for positive regulation of the expression of several hyphal-specific genes. sap5, which encodes secreted aspartic proteinase, is one of the mentioned genes and is crucial for pathogenicity properties. in the present work we have established the exper...

Background: Bone marrow stromal cells (BMSC) have been successfully employed for movement deficit recovery in spinal cord injury (SCI) rat models. One of the unsettled problems in cell transplantation is the relative high proportion of cell death, specifically after neural differentiation. According to our previous studies, p75 receptor, known as the death receptor, is only expressed in BMSC in...

The purpose of this study was to measure daily and seasonal evapotranspiration and daily crop coefficient of two common varieties of sunflower (Sirna and Euroflor) via drip-tape irrigation system. For this purpose, the sunflower water use was determined by daily monitoring of soil moisture at the depths of 10, 20, 30, 40 and 60 cm, and the crop evapotranspiration (ETC) was measured using volume...

Objective(s): siRNA may be a very promising tool for treatment of various diseases especially in cancer therapy due to high specificity. One of the main hurdles applications of siRNAs in vivo is optimization of the delivery strategy, especially the carrier systems. The aim of this study was to optimize siRNA delivery into suspended erythroleukemic cell line K562. Materials and Methods: We appli...

background: breast cancer is the most common diagnosed cancer among women in the world. snail1 plays a role in the development of the invasive phenotypes of cancer, neural cell differentiation, cell division and apoptosis in tumor cells. traces of snail1 in metastasis of breast cancer to bone are observed. the aim of this study was to investigate the effect of specific snail1 sirnas on the prol...

objective(s): sirna may be a very promising tool for treatment of various diseases especially in cancer therapy due to high specificity. one of the main hurdles applications of sirnas in vivo is optimization of the delivery strategy, especially the carrier systems. the aim of this study was to optimize sirna delivery into suspended erythroleukemic cell line k562. materials and methods: we appli...

The signal transduction of human programmed death ligand 1 (PD-L1), as an important checkpoint gene in the immune system, plays a key role internal function and survival cancer cells. In this paper, PD-L1-siRNA immunostimulatory IL-2 encoding plasmid DNA loaded by polyethylenimine (PEI) lipid nanoparticles (PEI-LNPs) were constructed. Its therapeutic effect on lung was evaluated, its physical p...

Gene therapy aims to silence an oncogene through RNA interference, or replace abnormal tumour suppressor via gene augmentation. In this study, we intended interference for PRKCA and augmentation PTEN with a view reduce growth in mouse model of breast cancer. Inorganic carbonate apatite nanoparticles (CA NPs) were utilized deliver the synthetic siRNA purified gene-carrying plasmid DNA both vitro...

despite the introduction of new antifungal agents, resistances to antifungal therapy continue to increase and outcome of invasive fungal infections treatment is frequently suboptimal. a large amount of the recent effort in antifungal drug discovery has focused on a limited set of targets with functions known or expected to be important for fungal viability and virulence. a variety of techniques...