BACKGROUND The advancement of gene silencing via RNA interference is limited by the lack of effective short interfering RNA (siRNA) delivery vectors. Rational design of polymeric carriers has been complicated by the fact that most chemical modifications affect multiple aspects of the delivery process. In this work, the extent of primary amine acetylation of generation 5 poly(amidoamine) (PAMAM)...

Successful delivery of irf5-siRNA by PEI-LBL-NCs resulted macrophages phenotype change from the M1 to M2 and accelerated wound healing.

Despite the great therapeutics potential, sustained release of small interfering RNA (siRNA) remains a challenge. Development siRNA nanoparticle provides possibility to further enhance therapeutic efficacy in gene-based therapy. Herein, we present new system based on encapsulation siRNA/chitosan-methacrylate (CMA) complexes into liposomes form UV crosslinkable Nanolipogels (NLGs) with siRNA-rel...

Polycationic nanocarriers attract increasing attention to the field of siRNA delivery. We investigated the self-assembly of siRNA vs pDNA with polycations, which are broadly used for nonviral gene and siRNA delivery. Although polyethyleneimine (PEI) was routinely adopted as siRNA carrier based on its efficacy in delivering pDNA, it has not been investigated yet why PEI efficiently delivers pDNA...

In this study, novel peptide-targeted delivery systems were developed for systemic and targeted delivery of therapeutic siRNA based on a multifunctional carrier, (1-aminoethyl)iminobis[N-(oleicylcysteinylhistinyl-1-aminoethyl)propionamide] (EHCO), which showed pH-sensitive amphiphilic cell membrane disruption. EHCO formed stable nanoparticles with siRNA. Targeted siRNA delivery systems were rea...

Bmi1 gene overexpression is found in various human tumors and has been shown as a potential target for gene treatment. However, siRNA-based treatments targeting Bmi1 gene have been restricted to limited delivery, low bioavailability and hence relatively reduced efficacy. To overcome these barriers, we developed a folate receptor targeted co-delivery system folate-doxorubicin/Bmi1 siRNA liposome...

background: introduction of the rna interference (rnai) machinery has guided the researchers to discover the function of essential vital or virulence factor genes in the microorganisms such as fungi. in the filamentous fungus aspergillus nidulans, the gene sidb plays an essential role in septation, conidiation and vegetative hyphal growth. in the present study, we benefited from the rnai strate...

RNA interference (RNAi) is a gene regulation mechanism initiated by RNA molecules that enables sequence-specific gene silencing by promoting degradation of specific mRNAs. Molecular therapy using small interfering RNA (siRNA) has shown great therapeutic potential for diseases caused by abnormal gene overexpression or mutation. The major challenges to application of siRNA therapeutics include th...

Abstract Background To date, triple-negative breast cancer (TNBC) treatment options are limited because of the loss target receptors and, as a result, only managed with chemotherapy. What is worse that TNBC frequently developing resistance to By using small interfering RNA (siRNA)-based therapeutics, our recent work demonstrated X-box-binding protein 1 (XBP1) was linked human epidermal growth f...

Small interfering RNA (siRNA) can be used to suppress gene expression, thereby providing a new avenue for the treatment of various diseases. However, the successful implementation of siRNA therapy requires the use of delivery platforms that can overcome the major challenges of siRNA delivery, such as enzymatic degradation, low intracellular uptake and lysosomal entrapment. Here, a protocol for ...