The formation of electrostatic interactions between polyanionic siRNA and polycations gives an easy access to the colloidal particles capable delivering in vitro or vivo. Among used for delivery, chitosan occupies a special place due its unique physicochemical biological properties. In this chapter we describe fundamental practical aspects complexes siRNA. basis complexation oppositely charged ...

Therapeutics based on small interfering RNA (siRNA) have a great clinical potential; however, delivery problems have limited their clinical efficacy, and new siRNA delivery vehicles are greatly needed. In this report, we demonstrate that submicron particles (800-900 nm) composed of the polyketal PK3 and chloroquine, termed as the PKCNs, can deliver tumor necrosis factor-alpha (TNF-alpha) siRNA ...