نتایج جستجو برای: six primer pairs (pb
تعداد نتایج: 475275 فیلتر نتایج به سال:
Despite the widespread prevalence of canine parvovirus disease (CPV) in Iranian dog population,molecular diagnosis of CPV variants, and investigation of the trends of its genetic changes is a new effort. Inthis study 50 samples from dogs suspicious of infection with clinical signs of diarrhea and vomiting, and 25samples from dogs suspected of infection with general symptoms su...
Backgrounds: Canine parvovirus (CPV) has been incriminated as a primary pathogen related to acute hemorrhagic enteritis in dogs. Three major antigenic variants of CPV (CPV-2a/2b/2c) have so far been identified. Objectives: This study was carried out to investigate the frequency of CPV-2 and its variants (CPV-2a/2b/2c) in a population of healthy and diarrheic dogs in the north west of Iran. Meth...
The purpose of this study was to evaluate the microtensile bond strength (MTBS) of an experimental adhesive system, which was prepared using different concentrations of calcium chloride (CaCl(2)) and synthetic peptides (pA/pB). Specimens were divided into six experimental groups and two control groups. In the experimental groups, self-etching primers used in the adhesive system comprised both P...
the genus artemisia containing more than 500 taxa at the specific or subspecific level. thirty-four species of genus artemisia have been reported in iran, which from the viewpoint distribution, density and coverage is the most important genera of plant. since the still has not been achieved a general system for the classification and phylogeny, in this research tried with evaluation of chloropl...
UNLABELLED PREMISE OF THE STUDY Microsatellite loci were developed for Prunus sibirica to investigate genetic diversity, population genetic structure, and marker-assisted selection of late-blooming cultivars in the breeding of P. sibirica. • METHODS AND RESULTS Using a magnetic bead enrichment strategy, 19 primer pairs were developed and characterized across 40 individuals from three P. si...
Thirty eight ecotypes of watermelon were collected from different parts of Iran. After the preparation of the field, these eotypes were cultivated in a completely randomized block design with three replications. In order to invest-igate genetic diversity, genomic DNA samples were extracted from leaves and Polymerase chain reactions were optimized using 14 SRAP primer pairs. One hundred thirty s...
Real-time quantitative polymerase chain reaction (qPCR) is one of the most important methods for analyzing the expression patterns of target genes. However, successful qPCR experiments rely heavily on the use of high-quality primers. Various qPCR primer databases have been developed to address this issue, but these databases target only a few important organisms. Here, we developed the qPrimerD...
AIMS To screen a pair of primers suitable for denaturing gradient gel electrophoretic (DGGE) analysis of ruminal methanogenic Archaea and to detect the archaeal communities in the rumen of goat. METHODS AND RESULTS Nine primer pairs for 16S rDNA of methanogenic Archaea, including six for directed polymerase chain reaction (PCR) and three for nested PCR were first evaluated by PCR amplificatio...
Accurate DNA replication is essential for genomic stability. One mechanism by which high-fidelity DNA polymerases maintain replication accuracy involves stalling of the polymerase in response to covalent incorporation of mismatched base pairs, thereby favoring subsequent mismatch excision. Some polymerases retain a "short-term memory" of replication errors, responding to mismatches up to four b...
Sequence characterized amplifled region (SCAR) markers were derived from random amplified polymorphic DNAs (RAPDs) that segregate in a longleaf pine x slash pine Fi family. Nine RAPD fragments, five from longleafpine and four from slash pine, were cloned and end sequenced. A total of 13 SCAR primer pairs, with lengths between 17 and 24 nucleotides, were developed. Nine (for SCAR loci FGPO04, FG...
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