Permafrost stores approximately 50% of global soil carbon (C) in a frozen form; it is thawing rapidly under climate change, and little is known about viral communities in these soils or their roles in C cycling. In permafrost soils, microorganisms contribute significantly to C cycling, and characterizing them has recently been shown to improve prediction of ecosystem function. In other ecosyste...

Pectinases are industrially important enzymes. The aim of this work was the isolation of pectinases producing Bacillus species from soil sample and their identification by phenotypic tests as well as production and purification pectinases enzyme. Isolated bacteria Subjected to various screening activity and gives pectinases screening test positive i.e. bacteria produced pectinases enzyme. The e...

The ecological forecasting of microorganisms and water quality dynamics in Krasnoyarsk reservoir is based on knowledge of dependence of specific growth rate (SGR) on limiting substrata. It investigated the influence of soil extract on the growth autochtonic bacterioplankton of Krasnoyarsk reservoir. The maximum SGR and Michaelis-Menten constant (on chemical oxidation decomposition (COD) permang...

Although it is widely recognized that microorganisms are essential for sustaining soil fertility, structure, nutrient cycling, groundwater purification, and other soil functions, soil microbial toxicity data were excluded from the derivation of Ecological Soil Screening Levels (Eco-SSL) in the United States. Among the reasons for such exclusion were claims that microbial toxicity tests were too...

BACKGROUND Escherichia coli is one of the most common pathogenic bacteria, which cause urinary tract infections in infants as well as in adult human beings. Due to the emergence of antibiotic resistance in E. coli, there is a great demand of new antimicrobial agent for the treatment of infections caused by such E. coli. This study aims to isolate, identify and characterize the native soil-bacte...

A pre-lysis buffer washing procedure was introduced to DNA extraction from a forest soil with high organic matter and iron oxide contents. Sodium phosphate of 0.1 M (pH 7.5) was used as a buffer to wash soil samples when subsequent lysis buffer was phosphate, and 20 mM EDTA (pH 7.5) was used when subsequent lysis buffer included EDTA. Initial experiments were not successful because the DNA extr...

The histone-like protein HU is the most-abundant DNA-binding protein in bacteria. The HU protein non-specifically binds and bends DNA as a hetero- or homodimer, and can participate in DNA supercoiling and DNA condensation. It also takes part in DNA functions such as replication, recombination, and repair. HU does not recognize any specific sequences but shows a certain degree of specificity to ...