OBJECTIVE(S) The present study reports the development and validation of a sensitive and rapid extraction method beside high performance liquid chromatographic method for the determination of crocetin in human serum. MATERIALS AND METHODS The HPLC method was carried out by using a C18 reversed-phase column and a mobile phase composed of methanol/water/acetic acid (85:14.5:0.5 v/v/v) at the fl...

Solid phase extraction (SPE) or liquid-solid extraction is poplar and growing techniques that areused to sample preparation for analysis. Metal nanoparticles have been studied for decades, and this is due to the properties that these materials do not have in bulk state. In nanoscale materials, the numbers of surface atoms is high and hence, have significant surface effects. A novel, simpl...

Biological sample pretreatment is an important step in biological sample analysis. Due to the diversity of biological matrices, the analysis of target substances in these samples presents significant challenges to sample processing. To meet these emerging demands on biopharmaceutical analysis, this paper summarizes several new techniques of on-line biological sample processing: solid phase extr...

A review of the many uses of matrix solid phase dispersion (MSPD) in the extraction and analysis of a variety of compounds from a range of samples is provided. Matrix solid phase dispersion (MSPD) has found particular application as a somewhat generic analytical process for the preparation, extraction and fractionation of solid, semi-solid and/or highly viscous biological samples. Its simplicit...

Background & Aim: Essential oils are isolated from different sections of plants such as flowers, seeds, leaves, stems and roots. Essential oils are applied extensively in producing of perfumes, cosmetics, foods, beverages and confectioneries and as ingredients of disinfectants and insecticides. Experimental: Essential oil are obtained by several isolation methods, such as hydrodistillation (HD...

Solid phase extraction (SPE) and supercritical fluid extraction (SFE) have become established in the field of analytical chemistry over the last five years. Both techniques are alternatives to classical analytical extraction methods used to separate analytes from solid and liquid matrices. SFE is an alternative to traditional liquid-solid extraction methods, the two most common being soxhlet an...

Phthalate esters are endocrine disrupters or mutagens. They are widely used as plasticizers and can be usually found in environmental samples, such as food, soil and polluted air. However, it is difficult to directly determine phthalate esters owing to their relatively low concentration and complex matrices. Therefore, preconcentration and separation have become increasingly important. In recen...