In this study a sensitive, simple and accurate spectrophotometric method was suggested for determination of tamsulosin in bulk powder and pharmaceutical dosage form based on the formation of an ion-pair complex between the drug and bromocresol green in a buffer solution at pH 3.5. The formed yellow color complex was extracted with chloroform and measured at 415 nm. The optimum reaction conditio...

The aim of this work was to develop a new and simple coacervative extraction method for the preconcentration and spectrophotometric determination of Cu(II) in water samples. Dithizone was used as the chelating agent while an anionic surfactant, namely sodium dodecyl sulfate (SDS), was used as extracting agent at room temperature. Central composite design (CCD) based on response surface methodol...

In this work the possibility of simultaneous spectrophotometric determination of chromium (VI) and iron (III) in alloys with help of the mixed organic reagents (diphenylcarbazide and 1,10-phenanthroline, diphenylcarbazide and sulphosalicylic acid) is studied. We have applied curve fitting method to separate individual signals of chromium (VI) and iron (III) from overlapped absorption spectrum. ...

Spectrophotometric or colorimetric data are normally obtained in some type of photometric unit and must be dealt with mathematically to provide a final result expressedin concentration. Such mathematical operationsare time-consuming, require data transfer, and are subject to human error. A photometric systemhas been devised which can be adjusted to perform calculations automatically and provide...

A simple, accurate and sensitive spectrophotometric method for the determination of Procaine penicillin (PP) is described. The method is based on charge-transfer reaction of PP with metol (N-methyl-p-hydroxy aniline) in the presence of ferric sulphate to form a purplewater soluble complex ,which is stable and has a maximum absorption at 510 nm .A graph of absorbance versus concentration shows t...

The enantioselectivity of epoxide hydrolase from Agrobacterium radiobacter (EchA) was improved using error-prone PCR and DNA shuffling. An agar plate assay was used to screen the mutant libraries for activity. Screening for improved enantioselectivity was subsequently done by spectrophotometric progress curve analysis of the conversion of para-nitrophenyl glycidyl ether (pNPGE). Kinetic resolut...

Two stability indicating UV-spectrophotometric methods were developed and validated for the determination of repaglinide with its degradation products. Assay of repaglinide in 0.1M NaOH and 0.1M HCl were achieved at 216nm and 243nm respectively. The correlation was in the range of 1.0 – 25 μg mL (r = 0.9999) for 0.1M NaOH and 2.0 – 40 μg mL (r = 0.9999) for 0.1M HCl with molar absorptivity valu...

In normal Amount Total Error of recovery Method plasma added found (%) Spectrophotometric 079 0-5 1*28 -3-4 1*0 0.4 1*41 +2-5 -1*0 1.0 1*96 -4.0 -0-82 0-7 1-53 +1*4 -0*82 1*2 2*18 -1*7 Photoelectric 076 0 5 1F24 -4 0 -1-03 0-4 1*43 +1'2 -1-03 1.0 2*0 -3*0 -0'84 0 7 1*56 +2-8 -0*84 1*2 2*01 -2.5 Roe & Kuether (1943) -0-72 0 5 1-2 -4 0 -1.0 0.4 1*4 0.0 -1.0 1.0 1*96 -4*0 -0*88 0*7 1*6 +2-8 -0*88 ...

Gabapentin is an anticonvulsant widely used in the treatment of epilepsy. No peculiar chromophore is available on the gabapentin moiety for direct analysis by absorption spectrophotometry. A sensitive spectrophotometric method for the determination of gabapentin in bulk, pharmaceutical formulations and human plasma has been developed. In this method, gabapentin directly derivatized with vanilli...

Fucoxanthin is a natural pigment found in microalgae, especially diatoms and Chrysophyta. Recently, it has been shown to have anti-inflammatory, anti-tumor, and anti-obesityactivity in humans. Phaeodactylum tricornutum is a diatom with high economic potential due to its high content of fucoxanthin and eicosapentaenoic acid. In order to improve fucoxanthin production, physical and chemical mutag...