The glass surface modification with 3-(mercaptopropyl) trimethoxysilane (MPTS), gold nano-particles (GN) and guanine rich single strand DNA (ss-DNA) was utilized as a novel and efficient platform for sensing trace concentration of methylene blue (MB) by an inexpensive spectrophotometric method. Methylene Blue (MB) can interact with the guanine base of single strand DNA and absorbed onto glass s...

Recently solution scanometry, as a new, simple, fast, portable, and economic method was introduced for determination of acidity constants of indicator and dyes. In this study, a method was applied for the determination of the acidity constant of p-Rosolic acid (RA) and Bromoxylenol blue (BXB) indicators. The method is based on scanning of Plexiglas® sheet containing colored solutions with a fla...

The study involves the development of a simple, rapid, selective, and low-cost spectrophotometric method for determination ceftriaxone as pure in its pharmaceutical preparations. (CF) was diazotized by sodium nitrite NaNO2 solution presence hydrochloride acid HCl ice bath at range 0 to 5 ºC form diazonium salt, then coupled with 4,5-diphenylimidazole reagent (4,5-DPI) basic hydroxide purple col...

A new assay for 3-hydroxy-3-methylglutaryl CoA reductase (mevalonate: NADP oxidoreductase [acylating CoA] , EC 1.1.1.34) is based upon the measurement of released coenzyme A (SH) during the reduction of 3-hydroxy-3-methylglutaryl CoA to mevalonate. Coenzyme A was measured in the presence of dithiothreitol, required for activity, by reaction with 5,5’-dithiobis(2-nitrobenzoic acid). Sodium arsen...

In a previous publication (1) from this laboratory, it was demonstrated that the transamination of amino acids to a-ketoglutaric acid is a reaction of wide scope. Although the data reported in the earlier paper permitted the computation of Q transaminase values, these values were of limited quantitative significance since no attempt was made to study the kinetics of these reaction systems, the ...

a simple, selective and sensitive method for the determination of trace amount of titanium (iv) based on its catalytic effect on methylene blue- ascorbic acid redox reaction has been reported. the reaction was followed spectrophotometrically by measuring the change in absorbance of methylene blue at 665 nm, 5 minutes after the initiation of the reaction. in this study experimental parameters we...

Chymotrypsin is an important proteolytic enzyme in the human digestive system that cleaves milk proteins through hydrolysis reaction, making it interesting subject to study activity of proteases. In this work, we compared detection chymotrypsin by spectrophotometric dynamic light scattering (DLS) and quartz crystal microbalance (QCM) methods determined limit (LOD), 0.15 ± 0.01 nM for spectropho...

ABSTRACT: Two analytical methods were developed, one utilizing sensitive UPLC-MS/MS method while other being the spectrophotometric exploiting Dushman reaction. Both validated based on ICH guidelines and successfully applied to quantitate drug in marketed formulation. responded linearly range of 1.6 µgmL-1-6.4 µgmL-1 procedure followed Beer’s law concentration 30-100 µgmL-1. has been found have...