Forecasting is a strategy for delivering bad news and is compared to two other strategies, stalling and being blunt. Forecasting provides some warning that bad news is forthcoming without keeping the recipient in a state of indefinite suspense (stalling) or conveying the news abruptly (being blunt). Forecasting appears to be more effective than stalling or being blunt in helping a recipient to ...

During S-phase replication forks can stall at specific genetic loci. At some loci, the stalling events depend on the replisome components Schizosaccharomyces pombe Swi1 (Saccharomyces cerevisiae Tof1) and Swi3 (S. cerevisiae Csm3) as well as factors that bind DNA in a site-specific manner. Using a new genetic screen we identified Mrc1 (S. cerevisiae Mrc1/metazoan Claspin) as a replisome compone...

Specific nascent peptides in the ribosome exit tunnel can elicit translation arrest. Such ribosome stalling is used for regulation of expression of some bacterial and eukaryotic genes. The stalling is sensitive to additional cellular cues, most commonly the binding of specific small-molecular-weight cofactors to the ribosome. The role of cofactors in programmed translation arrest is unknown. By...

Members of the Erm methyltransferase family modify 23S rRNA of the bacterial ribosome and render cross-resistance to macrolides and multiple distantly related antibiotics. Previous studies have shown that the expression of erm is activated when a macrolide-bound ribosome stalls the translation of the leader peptide preceding the cotranscribed erm Ribosome stalling is thought to destabilize the ...

ion for algorithm design and programming, while LogP provides better control of machine resources. Comparisons and evaluations between the two models have been made in [29,62,104]. In [29], a variant of LogP that disallows the so-called stalling behavior is considered. The authors create cross simulations between the two models showing their equivalence in algorithmic design when using asymptot...

E xtracellular receptors anchored in the plasma membrane outer leaflet somehow entice intracellular proteins to the cytosolic side of the membrane for activation. Companion studies by Suzuki et al. (pages 717 and 731) give a peek at these dynamics at the level of single molecules. The team shows that liganded clusters of the GPI-anchored receptor (GPI-AR) CD59 undergo temporary immobiliza-tions...

Sproull et al.[1] to demonstrate the concept of asynchronous circuits. This architecture relies on distributed decision making and localized clocking and data movement. We have taken these ideas and refor-mulated them into a substantially faster more scalable architecture that has the same distributed decision making and locality for clock-ing and data, but adds very aggressive speculation, no ...

RNA-dependent RNA polymerases (RdRP) form an important class of enzymes that is responsible for genome replication and transcription in RNA viruses and involved in the regulation of RNA interference in plants and fungi. The RdRP kinetics have been extensively studied, but pausing, an important regulatory mechanism for RNA polymerases that has also been implicated in RNA recombination, has not b...