Many proteins that accumulate in the form of insoluble aggregates when they are overproduced in Escherichia coli can be rendered soluble by fusing them to E. coli maltose binding protein (MBP), and this will often enable them to fold in to their biologically active conformations. Yet, although it is an excellent solubility enhancer, MBP is not a particularly good affinity tag for protein purifi...

le clezio 's fictional world takes us back to the beauties of nature and makes us contemplate the mysteries of the universe. by creating exaggerated and sometimes surreal sceneries, le clezio seeks to escape from inconveniences of the materialistic world to explore man's identity. obscure conditions, wearriness with the achievements of modern world, le clezio's personages, are en...

Let G be a simple graph on d vertices. We define a monomial ideal K in the Stanley-Reisner ring A of the order complex of the Boolean algebra on d atoms. The monomials in K are in one-to-one correspondence with the proper colorings of G. In particular, the Hilbert polynomial of K equals the chromatic polynomial of G. The ideal K is generated by square-free monomials, so A/K is the Stanley-Reisn...

For an $n$-gon with vertices at points $1,2,cdots,n$, the Betti numbers of its suspension, the simplicial complex that involves two more vertices $n+1$ and $n+2$, is known. In this paper, with a constructive and simple proof, wegeneralize this result to find the minimal free resolution and Betti numbers of the $S$-module $S/I$ where  $S=K[x_{1},cdots, x_{n}]$ and $I$ is the associated ideal to ...

A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.

Fluorescent probes are essential for the exploration of protein function, detection of molecular interactions, and conformational changes. The nitrilotriacetic acid derivatives of different chromophores were successfully used for site-selective noncovalent fluorescence labeling of histidine-tagged proteins. All of them, however, suffer from the same drawback--loss of the fluorescence upon bindi...

Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, S...

I must admit that I had no prior knowledge of Richard Selzer or his work before reading this book. The title had the air of so many other trite books written by doctorsno doubt an attempt by the publishers to exploit the profession's mystique for dollar signs and, as such, I really wasn't expecting much another compilation of "let me tell you why I'm so great" stories is what I thought was awai...

I must admit that I had no prior knowledge of Richard Selzer or his work before reading this book. The title had the air of so many other trite books written by doctorsno doubt an attempt by the publishers to exploit the profession's mystique for dollar signs and, as such, I really wasn't expecting much another compilation of "let me tell you why I'm so great" stories is what I thought was awai...