Matrix metalloproteinase-9 is elevated within the acutely injured murine spinal cord and blockade of this early proteolytic activity with GM6001, a broad-spectrum matrix metalloproteinase inhibitor, results in improved recovery after spinal cord injury. As matrix metalloproteinase-9 is likewise acutely elevated in dogs with naturally occurring spinal cord injuries, we evaluated efficacy of GM60...

A novel practical preparation method for methyl methanethiosulfonate (MMTS) has been developed. Dimethyl sulfoxide was converted to MMTS in the presence of a catalytic amount oxalyl chloride or ...

Incubations with human liver and gut microsomes revealed that the antibiotic, clindamycin, is primarily oxidized to form clindamycin sulfoxide. In this report, evidence is presented that the S-oxidation of clindamycin is primarily mediated by CYP3A. This conclusion is based upon several lines of in vitro evidence, including the following. 1) Incubations with clindamycin in hepatic microsomes fr...

Moskovitz J, Du F, Bowman CF, Yan SS. Methionine sulfoxide reductase A affects -amyloid solubility and mitochondrial function in a mouse model of Alzheimer’s disease. Am J Physiol Endocrinol Metab 310: E388–E393, 2016. First published January 19, 2016; doi:10.1152/ajpendo.00453.2015.—Accumulation of oxidized proteins, and especially -amyloid (A ), is thought to be one of the common causes of Al...

The potential roles of human hepatic and renal flavin-containing monooxygenases (FMOs) in the metabolism of the cysteine S-conjugates S-allyl cysteine (SAC) and S-(1,2-dichlorovinyl)-L-cysteine (DCVC) were investigated. Incubations of human cDNA-expressed FMO1, FMO3, FMO4, and FMO5 with SAC resulted in detection of SAC sulfoxide, with FMO3 exhibiting approximately 3-, 4-, and 10-fold higher act...

Primary bovine embryonic kidney cells frozen with dimethyl sulfoxide can be recultured with satisfactory results.