Localized surface plasmon resonance (LSPR) is strongly associated with inelastic scattering of biochemical molecules near metal nanostructures, i.e., surface enhanced Raman scattering (SERS). Systematically exploration of the relationship between surface-enhanced Raman spectroscopy (SERS) and the plasmon resonance wavelength has been an experimental limitation due to the lack of reliable and tu...

4. LIGAND 10 4.1. Direct versus indirect immobilisation 10 4.2. Covalent immobilisation 11 4.2.1. A general approach 11 4.2.2. Choice of chemistry 12 4.2.3. Prepare the protein 12 4.2.4. Pre-concentration 12 4.2.5. Amine coupling 14 4.2.6. Regeneration 15 4.2.7. Adjusting the immobilisation conditions. 16 4.3. Non-covalent immobilisation (ligand capture) 16 4.3.1. Using an existing strategy 17 ...

Available online 20 November 2014