The determination of the thickness and dielectric constant of thin dielectric layers by means of surface plasmon resonance is discussed. It appears to be impossible to determine these parameters from one surface plasmon response experiment. This is illustrated theoretically. Variation of the refractive index of the solution in which surface plasmon experiments were performed allowed us to deter...

OpenSPRTM is a powerful instrument providing indepth label-free binding kinetics for a variety of different molecular interactions. With any new technology, it is important to compare the performance of various tools and techniques to establish their consistency and accuracy. In this study, binding kinetic results of protein-protein interactions are compared between the OpenSPRTM, BiacoreTM and...

The recent work reporting the observation of the increase in sensitivity for surface plasmon resonance (SPR) spectroscopy at smaller incident angles, is further studied with variation in the temperature of the SPR sensor. Using a simple theoretical model established previously, it is found that in spite of the increase in damping for the resonance at higher temperatures, the recent finding of t...

A sensing method based on resonance localized surface plasmon spectroscopy was developed for low molecular weight substrate and inhibitor molecules binding to heme proteins. Cytochrome P450 proteins have Soret and Q absorption bands in the visible wavelength region. The coupling between the molecular resonance of P450 and the localized surface plasmon resonance (LSPR) of functionalized silver n...

Electron energy-loss spectroscopy can be used for detailed spatial and spectral characterization of optical excitations in metal nanoparticles. In previous electron energy-loss experiments on silver nanoparticles with radii smaller than 20 nm, only the dipolar surface plasmon resonance was assumed to play a role. Here, applying electron energy-loss spectroscopy to individual silver nanoparticle...

The propagation of a plasmon surface wave in deep metallic lamellar gratings is shown to be characterized by absorption losses smaller than on a flat metallic-dielectric interface. This feature is due to the formation of a resonance of the electric field inside the groove. Similar to the plasmon surface wave in shallow gratings, this kind of plasmon can lead to total absorption of incident ligh...

Surface plasmon resonances on bilayer aluminum nanowire gratings are studied in both theory and experiment. It is found that there are two kinds of surface plasmon on the bilayer metallic gating: longitudinal aluminum/dielectric/aluminum slit and lateral aluminum/dielectric interface waveguide mode. The surface plasmon waveguide mode resonance in the slits makes the grating act as a transverse ...

Structured films with periodic arrays of nanoholes covered by half-cone shells are fabricated via a simple and efficient colloidal lithography method. The designed films show strong polarization dependence in optical transmission. By decreasing the height of half-cone shells the peak shifts and this shift varies strongly for different orthogonal polarizations. Furthermore, the three-dimensional...

The plasmonic coupling between gold caps and holes in thin films was investigated experimentally and through finite-difference time-domain (FDTD) calculations. Sparse colloidal lithography combined with a novel thermal treatment was used to control the vertical spacing between caps and hole arrays and compared to separated arrays of holes or caps. Optical spectroscopy and FDTD simulations revea...

The Silver SPR chip was modified by alkaline-silane condensation with aminopropyltriethoxysilane (APTES) in NaOH aqueous solution at different times. Silver sputtered slides coated with APTES were immersed in NaOH solution, enabling us to produce silver surfaces homogeneously covered with APTES. The surface properties of grafted APTES on sputtered silver surface as a occasion of time were studi...