The objectives of this study were to investigate the origin of highly discordant rifampin (rifampicin) (RMP) drug susceptibility test results obtained for Mycobacterium tuberculosis strains during proficiency testing. Nine Supra-National Tuberculosis Reference Laboratories tested the RMP susceptibilities of 19 selected M. tuberculosis strains, using standard culture-based methods. The strains w...

Standard reference strains of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were suspended in sterile deionized water and tested daily via disk agar diffusion for their antimicrobial susceptibilities. Inocula of E. coli and P. aeruginosa yielded acceptable results for up to 32 days; however, results on S. aureus were unacceptable due to loss of viability of the organism in...

A simple, abbreviated broth dilution test (tube test) utilizing a commercially available medium and inexpensive disposable materials, and which could be performed entirely in room air, was developed and used to test the susceptibility of 100 strains of anaerobic bacteria to clindamycin, chloramphenicol, ampicillin, and tetracycline. Results are reported in categories of susceptibility: suscepti...

We collected 11 groups of Aedes aegypti mosquitoes from various locations of Thailand. After rearing in the laboratory, the colonies were tested for KT50 values to dl,d-T80-allethrin 0.5% mosquito coils in a 25 m3 room semi-field test and KD50 and LD50 values to dl,d-T80-allethrin by a topical application method. Two groups of mosquitoes were susceptible to allethrin similar to a SS (known alle...

How-foo Chen1, Chi-Hung Lin2,3,4, Chun-Yao Su1, Hsin-Pai Chen5 and Ya-Ling Chiang1 1Institute of Biophotonics, National Yang Ming University, Taipei 2Institute of Microbiology & Immunology, National Yang Ming University, Taipei 3Taipei City Hospital 4Department of Surgery, Veteran General Hospital, Taipei 5Department of Medicine, National Yang-Ming University Hospital, Yilan, Taiwan and School ...

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BACKGROUND Drug susceptibility testing for Mycobacterium tuberculosis (M. tuberculosis) is especially required in difficult cases of tuberculosis (TB) chemotherapy and in cases of multidrug resistance (MDR-TB; combined resistance to isonizid and rifampicin with or without resistance to any other drug). The methods for in vitro cultivation and drug susceptibility testing (DST) of M. tuberculosis...

Concentrations of 20 mug of sulfadiazine or 30 mug of d-cycloserine/ml were found to be suitable for testing the differential susceptibility of Chlamydia trachomatis and C. psittaci to these drugs in cell cultures.

BACKGROUND Fluoroquinolones and second-line injectable drugs are the backbone of treatment regimens for multidrug-resistant tuberculosis, and resistance to these drugs defines extensively drug-resistant tuberculosis. We assessed the accuracy of an automated, cartridge-based molecular assay for the detection, directly from sputum specimens, of Mycobacterium tuberculosis with resistance to fluoro...