نتایج جستجو برای: suspending medium
تعداد نتایج: 237712 فیلتر نتایج به سال:
MUSTARD,, J .FRASER,DENNIS W .YERRY, RAELENE L. KINLOUGHRATHBONE, AND MARIAN A. PACKHAM. Factors resfonsible for ADPinduced release reaction of human platelets. Am. J. Physiol, 228(6) : 17571765. 1975,-Extensive aggregation of human platelets can be induced by ADP without secondary aggregation or release of granule contents. This occurs with washed platelets in Tyrode solution containing 0.35 Y...
A novel method, free flow acoustophoresis (FFA), capable of continuous separation of mixed particle suspensions into multiple outlet fractions is presented. Acoustic forces are utilized to separate particles based on their size and density. The method is shown to be suitable for both biological and nonbiological suspended particles. The microfluidic separation chips were fabricated using conven...
The interactions of normal erythrocytes and erythrocytes from patients having hemoglobin S hemoglobinopathies with normal human endothelial cells (EC) were investigated under flow conditions. When EC supernatant, containing 2.8-11.0 U/dl of von Willebrand factor (vWF) antigen and vWF multimeric forms larger than those present in normal plasma, was the red blood cell (RBC)-suspending medium inst...
Addition of beta-lapachone, an o-naphthoquinone endowed with trypanocidal properties to respiring Trypanosoma cruzi epimastigotes induced the release of O2- and H2O2 from the whole cells to the suspending medium. The same beta-lapachone concentration (4 micron) that released H2O2 at maximal rate completely inhibited T. cruzi growth in a liquid medium. The position isomer, alpha-lapachone, did ...
The shear flow of non-Brownian glass spheres suspended in a concentrated colloidal dispersion that exhibits non-Newtonian rheology is investigated. At low volume fractions, the addition of non-Brownian spherical particles to the colloidal dispersion leads to an increase in the shear viscosity as well as the dynamic moduli. The flow curves of these suspensions are qualitatively similar to the su...
When cells of a marine pseudomonad washed free of medium components with 0.05 m MgSO(4) were suspended in solutions containing 200-mm concentrations of various salts, there was an immediate increase in optical density (OD), followed by a slow decrease. The decrease following the initial increase, but not the increase itself, could be prevented by omitting K(+) from or by adding metabolic inhibi...
A single inlet two-stage acoustophoresis chip enabling tumor cell enrichment from white blood cells.
Metastatic disease is responsible for most cancer deaths, and hematogenous spread through circulating tumor cells (CTC) is a prerequisite for tumor dissemination. CTCs may undergo epithelial-mesenchymal transition where many epithelial cell characteristics are lost. Therefore, CTC isolation systems relying on epithelial cell markers are at risk of losing important subpopulations of cells. Here,...
The views that catabolism of protein leads to net production of acid and that urinary excretion of ammonium ion represents an equimolar excretion of proton are not compatible with basic chemical relationships (Atkinson, D. E., and Camien, M, N. (1982) Curr. Top. Cell. Regul. 21, 261-302). Metabolism of protein produces significant amounts of base (bicarbonate), which is disposed of in the synth...
any change in the refractive index of a laser active medium can lead to serious degradation of beam quality, laser beam modes, laser performance and variation in the intensity distribution. alteration in the refractive index of laser active medium is especially notable in high power lasers. it is clear that in the laser beam production, the pumping agent induces a great amount of heat which...
By FRANCES L. ESTES, SHIRLEY SMITH AND JOSEPH H. GAST I HE IN VITRO STUDY of normal leukocyte metabolism has been complicated by the presence of erythrocytes and platelets in the cell suspensions and by the need for a good yield of cells of reasonably constant characteristics. Because of the difficulties involved in recovering uncontaminated leukocytes of a single type from blood, a study of th...
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