Ruthenium red induces the agglutination of ascites hepatoma cells. The agglutination was inhibited by acidic polysaccharides but not by haptenic inhibitors of some plant agglutinins. The agglutination was also abolished by treatment of the cells with a low concentration of papain. Papain digestion causes a decrease in the number of microvilli on the cell surface and the release of carbohydrate-...

The period enforcer algorithm for self-suspending real-time tasks is a technique for suppressing the “backto-back” scheduling penalty associated with deferred execution. Originally proposed in 1991, the algorithm has attracted renewed interest in recent years. This note revisits the algorithm in the light of recent developments in the analysis of self-suspending tasks, carefully re-examines and...

Abstract We introduce a novel 3D microrheology system that combines for the first time Optical Tweezers with Integrated Multiplane Microscopy (OpTIMuM). The allows tracking of an optically trapped bead, ~ 20 nm accuracy along optical axis. This is achieved without need high precision z-stage, separate calibration sample, nor priori knowledge either bead size or properties suspending medium. Ins...

Previous experimentation involving the use of dispersed rat liver cells have utilized suspending media common to fractionation and slicing methods. Cells in these media have not remained viable for prolonged periods of time and they have resisted culturing techniques. Suspensions of dispersed parenchymal cells were prepared from rat livers which had been perfused in situ via the dorsal aorta wi...

background; musculoskeletal disorders (msds) are among the most prevalent pains which could be ignited via material handling. backpack wearers are among those who may carry heavy loads. since msds mainly originate in school-aged children, students (as the largest group of backpack wearers) are considered more important. the purpose of this study is to design a backpack based on minimization of ...

It has been shown that Krebs ascites tumor cells incubated in vitro with immune gamma globulin and complement lose the bulk of their cytoplasmic RNA to the suspending medium, although the cell membrane remains visibly intact. The present experiments show that about four-fifths of the lost RNA is sedimented by centrifugation of the cell-free medium at 105,000 g. Electron microscopic and chemical...

Isolation of proteins that specifically interact with a given RNA or RNA regulation element is essential for studies on the molecular mechanisms of gene expression. Here, a novel method for direct isolation of such interacting proteins is described. It uses an affinity medium that consists of an interacting RNA with an artificially added 'tail', which is annealed to one end of a DNA 'arm', the ...