Mixtures of powder and liquid are ubiquitous in nature as well industries exhibit complex flowing deforming behaviors, including sol to gel transition under shear stress. In order better understand the characteristic features this type mixture, we observed behavior a mixture colloidal silica particles water model system vibration. The showed different states, from powder-like viscous fluid-like...

Recebido para publicação em 25/11/2008 Aceito para publicação em 18/7/2009 (003957) 1 Animal Sciences, Universidade José do Rosário Vellano – UNIFENAS 2 Pharmacy Student, Universidade José do Rosário Vellano – UNIFENAS 3 Federal University of Alfenas – UNIFAL 4 Biology and Physiology Laboratory of Microorganisms, Universidade José do Rosário Vellano – UNIFENAS,Rod. MG, Km 179, CEP 37130-000, Al...

A simplified technique has been described for the continuous growth of mammalian cells in suspension culture. The cell population density increased as the rate of input of fresh medium was decreased, and the average generation time was concommittantly prolonged. At relatively high input rates, the population remained stabilized for an indefinite period, but at low flow rates, there was sometime...

A subline of 293 cells able to grow in suspension culture has been developed by passage of 293 cells through nude mice. This new line, designated 293N3S, grows with a doubling time of approximately 30 h, continues to express adenovirus 5 early region 1 (E1) antigens, and remains permissive for adenovirus 5 host range mutants defective in E1 functions.