To identify volatile constituents in Typhonium giganteum Engl., and clarify their effects on proliferation of breast cancer T47D cell lines. Volatile constituents in Typhonium giganteum Engl. are extracted by steam distillation, and determined by GC-MS. 24, 48 and 72 h after treatment of T47D cells with different concentrations of Typhonium giganteum Engl. volatile (DJL-V) constituents, methyl ...

The transforming growth factor-B (TGF-B) superfamily of growth factors is responsible for a variety of physiologic actions, including cell cycle regulation. Activin is a member of the TGF-B superfamily that inhibits the proliferation of breast cancer cells. Activin functions by interacting with its type I and type II receptors to induce phosphorylation of intracellular signaling molecules known...

We previously described that type B retrovirus-like particles released from the human mammary carcinoma cell line T47D are pseudotypes and package retroviral RNA of different origins (W. Seifarth, H. Skladny, F. Krieg-Schneider, A. Reichert, R. Hehlmann, and C. Leib-Mösch, J. Virol. 69:6408-6416, 1995). One preferentially packaged retroviral sequence, ERV-MLN, has now been used to isolate the c...

Kanker merupakan jenis penyakit dengan prevalensi tertinggi yang dapat menyebabkan kematian, salah satunya kanker payudara. Pengobatan kemoterapi saat ini dilakukan banyak menimbulkan resiko terhadap penderitanya, misalnya resistensi, efek samping, daya efikasi semakin menurun. Model pengobatan efektif yakni mengkombinasikan agen kemopreventif sebagai komplementer, sehingga meminimalkan samping...

The transforming growth factor-beta (TGF-beta) superfamily of growth factors is responsible for a variety of physiologic actions, including cell cycle regulation. Activin is a member of the TGF-beta superfamily that inhibits the proliferation of breast cancer cells. Activin functions by interacting with its type I and type II receptors to induce phosphorylation of intracellular signaling molecu...

BACKGROUND Adrenomedullin is a secreted peptide hormone with multiple activities. Several reports have indicated that adrenomedullin may be involved in tumor survival, but this has not been directly shown. Here we evaluate the in vitro and in vivo effects of adrenomedullin overexpression in human breast cancer cells. METHODS The human breast cancer cell lines T47D and MCF7, both of which expr...

The risk of human breast cancer is concerned to cumulative exposure of the breast cells to endogenous estrogens. Strategies aiming at reducing the production of estrogens may be useful for the prevention of estrogens-related breast cancer. Several natural products with plant origin have the potential value as chemo-preventive or therapeutic agents in cancer. Flavonoids, the natural polyphenol c...

Scoparia dulcis Linn plays an important role in treatment because it contains active compounds that are proven to have a variety of activities, including cytotoxicity on various cancer cells. The objective this study is isolate and identify the cytotoxic ethyl acetate fraction dulcis, observe cell cycle inhibition induction apoptosis vitro, carry out molecular studies using silico studies. A ne...

OBJECTIVES Berberine, a naturally occurring isoquinoline alkaloid, has shown antitumor properties in some in vitro systems. But the effect of berberine on breast cancer has not yet been completely studied. In this study, we evaluated anticancer properties of berberine in comparison to doxorubicin. MATERIALS AND METHODS The antiproliferative effects of berberine and doxorubicin alone and in co...

Specific progesterone binding by cultured human breast carcinoma T47D, MCF-7, and ZR75-1 cells was decreased 25-40% by epidermal growth factor (EGF), with a 50% effective dose of 0.1 UMEGF. Studies with the soluble and paniculate fractions prepared after homogenization of T47D cells grown in glass roller bottles revealed equivalent EGFinduced decreases in progesterone binding to receptors in bo...