OBJECTIVES Point of care testing (POCT) contributes to diagnosis and monitoring with fast testing time and easily performed assays. We evaluated the Abaxis Piccolo Xpress point of care chemistry analyzer using the Liver Panel Plus discs for our pediatric patient population at Texas Children's Hospital. DESIGN AND METHODS Analytical performance was evaluated for precision and linearity using q...

BACKGROUND Metabolomics studies can quantitatively detect the dynamic metabolic response of living systems. OBJECTIVE To detect urinary metabolomics after hepatic ischemia/reperfusion (I/R) injury induced by the Pringle maneuver using gas chromatography-mass spectrometry (GC-MS). METHODS Male Sprague-Dawley rats (N = 80) were randomly divided into 4 groups (n = 20/group): sham operation, da...

Delta bilirubin (B delta), isolated from serum, has an absorption maximum near 440 nm and a molar absorptivity of 72,000 L mol-1cm-1 in either Tris HCl (0.1 mol/L, pH 8.5) or phosphate (0.13 mol/L, pH 7.4) buffer. This absorptivity exceeds by approximately 50% and 59%, respectively, that of unconjugated bilirubin in the same buffers. This finding suggests that substantial errors can be incurred...

AIM To explore the effectiveness for treating liver fibrosis by combined transplantation of bone marrow-derived endothelial progenitor cells (BM-EPCs) and bone marrow-derived hepatocyte stem cells (BDHSCs) from the liver fibrosis environment. METHODS The liver fibrosis rat models were induced with carbon tetrachloride injections for 6 wk. BM-EPCs from rats with liver fibrosis were obtained by...

Background: Primary biliary cirrhosis (PBC) is an autoimmune liver condition with relatively slow progression, leading to liver failure and death. We retrospectively investigated potential risk factors of liver-related mortality in patients with PBC. Methods: The data of patients with PBC at the First Affiliated Hospital of Zhengzhou University in China from 2007 to 2013 were included, specific...

OBJECTIVES This study aimed to establish a novel non-binding, reversible rat model of acute cholangitis of the severe type (ACST). MATERIALS AND METHODS Twenty-six rats were randomly divided into the sham-operated group (n=13) and the ACST group (n=13). All rats were intubated with a modified catheter through the external jugular vein. The ACST model was established by ligation of the distal ...

This study aimed to investigate the role of 5-lipoxygenase (5-LO) in acute liver failure (ALF) and changes in macrophage activation by blocking it. ALF was induced in rats by administration of D-galactosamine (D-GalN)/lipopolysaccharide (LPS). Rats were injected intraperitoneally with AA-861 (a specific 5-LO inhibitor), 24 hr before D-GalN/LPS administration. After D-GalN/LPS injection, the liv...

Objective. To evaluate the efficacy and safety of large dosage of Chishao in formulae for treatment of cholestatic hepatitis. Methods. The major databases (PubMed, Embase, Cochrane Library, Chinese Biomedical Database Wanfang, VIP medicine information system, and China National Knowledge Infrastructure) were searched until January 2014. Randomized controlled trials (RCTs) of large dosage of Chi...

Background: Measuring metabolic parameters in the blood has been an indispensable tool for assessing productive and health status of dairy cows more than 100 years. The values laboratory depend on various preanalytical, analytical postanalytical factors. most important preanalytical factors are sample transport time temperature, hemolysis, anticoagulant type, volume.Preanalytical can lead to re...

OBJECTIVE To assess the effect of sevoflurane anesthesia on hepatic function in morbidly obese versus non-obese patients undergoing abdominal surgeries. METHODS We prospectively evaluated the levels of the serum concentration of liver enzymes aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), gamma glutamyl transferase (GGT), alkaline phosphatase (A...