Dyskeratosis congenita (DC) is a genetic disorder of defective tissue maintenance and cancer predisposition caused by short telomeres and impaired stem cell function. Telomerase mutations are thought to precipitate DC by reducing either the catalytic activity or the overall levels of the telomerase complex. However, the underlying genetic mutations and the mechanisms of telomere shortening rema...

Telomeres are specialized structures of eukaryotic chromosomes that protect the tips chromosomes. prevent from losing base pair sequences at their ends, and also stop fusing together. Telomerase is an enzyme rebuilds telomeres maintains genomic stability by extending after each cell division. activity prevents cellular aging, known as senescence. Immortal lines can be created inducing expressio...

Telomerase plays a critical role in cancer, prompting the pursuit of various telomerase-based therapeutic strategies. One such strategy, telomerase interference, exploits the high telomerase activity in cancer cells and reprograms telomerase to encode "toxic" telomeres. To date, telomerase interference has been tested in human cancer cells xenografted into mice, an approach that does not recapi...

Telomerase activity and altered telomere length have been extensively studied in many kinds of malignant tumors for clinical diagnostic and/or prognostic utilities. In the present study, we investigated telomerase activity and telomere length in colorectal cancers and noncancerous colonic mucosa specimens in 100 patients between 1991 and 1996. To determine whether the level of telomerase activi...

Human telomerase is a ribonucleoprotein (RNP) enzyme, comprising protein components and an RNA template that catalyses telomere elongation through the addition of TTAGGG repeats. Telomerase function has been implicated in aging and cancer cell immortalization. We report a rapid and efficient one-step purification protocol to obtain highly active telomerase from human cells. The purification is ...

Background & Aims: The prevalence, incidence, and complications of heart disease increase with age. Green tea is an antioxidant which can prevent cellular senescence and cardiovascular diseases. Telomerase enzyme decreases with aging. Acute exhaustive exercise results in disturbances in the oxidative system and production of active oxygen species in the heart tissue. Thus, the present study aim...

Introduction: Telomeres are DNA portions that are located on the two ends of the chromosome. Telomeres play an important role in cellular life. Exercise is one of the factors that contributes to their control. The purpose of the present study was to investigate the effect of 8 weeks of high intensity interval training (HIIT) on telomere length and telomerase activity in non-athletic young men. ...

Telomerase maintains ends of eukaryotic chromosomes, telomeres. Telomerase loss results in replicative senescence and a switch to recombination-dependent telomere maintenance. Telomerase insufficiency in humans leads to telomere syndromes associated with premature ageing and cancer predisposition. Here we use yeast to show that the survival of telomerase insufficiency differs from the survival ...

Telomerase activity is known to be absent from most normal and well-differentiated tissues, although being detectable in the vast majority of malignant tumors. An increasing number of reports demonstrate that telomerase may be activated in benign tumors, such as adenomas. We have investigated a series of normal and neoplastic thyroid tissues for the presence of telomerase activity. As expected,...

Telomerase is a key participant in the telomere length maintaining system in eukaryotic cells. Telomerase RNA and protein reverse transcriptase subunits are essential for the appearance of active telomerase in vitro. Telomerase is active in many cancer types and is a potential target for anticancer drug development. Here we report a new approach for impairing telomerase function at the stage of...