was able to obtain large volumes of high potency material by batch cultivation in tanks, but did not report any yields. was sterilized at 118 to 122 C for 1 hr, cooled rapidly to 36 to 39 C, and inoculated immediately. Seed cultures of Clostridium tetani (Massachusetts strain) were maintained at -70 C in 1.0-ml amounts in glass ampoules. One or four of these ampoules were thawed and inoculated ...

Background: Tetanus caused by clostridium tetani, despite being a preventable disease, is still posing significant health hazard to developing countries like Pakistan. Objective: To determine in-hospital mortality among patients of severe adult tetanus presenting with bad prognostic factors.Material and Methods: This cross-sectional study was conducted at the Department Medicine, Medical Teachi...

The past decade has seen remarkable progress in our understanding of the scale global typhoid fever problem,1 alarming emergence south Asia extensively drug-resistant Salmonella enterica serovar Typhi (S Typhi) threatening effective treatment,2 and WHO prequalification a conjugate vaccine,3 new tool to prevent disease. In Lancet, Firdausi Qadri colleagues report analysis 18 months follow-up clu...

Human antibody to diphtheria toxin was analyzed with an ammonium sulfate radioimmunoassay. In 15 immune sera with titers of between 0.03 and 25 units/ml, the avidity was similar to that of U.S. standard antitoxin. The proportion of the post-immunization anti-diphtheria antibody specific for fragment A was variable, but in the 10 sera tested constituted a substantial fraction of the antibody. Ap...

In this issue of Clinical and Vaccine Immunology, Scobie and colleagues (H. M. Scobie et al., Clin Vaccine Immunol 23:546-554, 2016, http://dx.doi.org/10.1128/CVI.00052-16) report a nationwide serosurvey of tetanus immunity in >2,000 Cambodian women of child-bearing age to monitor progress toward maternal and neonatal tetanus elimination. This commentary discusses vaccines as interventions for ...

CONTEXT Increasing reports of pertussis among US adolescents, adults, and their infant contacts have stimulated vaccine development for older age groups. OBJECTIVE To assess the immunogenicity and reactogenicity of a tetanus-diphtheria 5-component (pertussis toxoid, filamentous hemagglutinin, pertactin, and fimbriae types 2 and 3) acellular pertussis vaccine (Tdap) in adolescents and adults. ...

The pediatric formulations usually have 3-5 times as much of the diphtheria component than what is in the adult/ adolescent formulation. Similarly, the formulations for adults/adolescents have around one third of acellular pertussis content than pediatric formulations. The amount of tetanus toxoid in each of the products remains almost equivalent. Dose reduction for diphtheria and pertussis is ...

Specific antibody deficiency (SAD) is a common antibody immunodeficiency defined as a poor antibody response to unconjugated pneumococcal polysccharides present in the 23valent pneumococcal vaccine (PPV23). Clinical manifestations of specific antibody deficiency include recurrent sinopulmonary infections, such as sinusitis, otitis media, bronchitis, and pneumonia. All immunoglobulin concentrati...