The effects of reduced doses of OvaprimTM (GnRHa + domperidone) on sperm release of Brycon orbignyanus and Prochilodus lineatus were evaluated. Furthermore, sperm quality was compared among fresh, equilibrated and postthaw samples. Males received a single and reduced dose of OvaprimTM (0.125 or 0.25 ml/kg); control males received pituitary extract (cPE; 3 mg/kg). Fresh sperm was evaluated for v...

A slow freezing/rapid thawing method for the cryopreservation of human oocytes has been employed using a sodium-depleted culture media. In 53 frozen egg-embryo transfer (FEET) cycles, a 60.4% survival rate post-thaw was obtained and a 62.0% fertilization rate following intracytoplasmic sperm injection. Overall pregnancy rates were 26.4% per thaw attempt, 30.4% per patient, and 32.6% per embryo ...

BACKGROUND Variability among stallions in terms of semen cryopreservation quality renders it difficult to arrive at a standardized cryopreservation method. Different extenders and processing techniques (such us colloidal centrifugation) are used in order to optimize post-thaw sperm quality. Sperm chromatin integrity analysis is an effective tool for assessing such quality. The aim of the presen...

Ice formation in the xylem sap produces air bubbles that under negative xylem pressures may expand and cause embolism in the xylem conduits. We used the centrifuge method to evaluate the relationship between freeze-thaw embolism and conduit diameter across a range of xylem pressures (Px) in the conifers Pinus contorta and Juniperus scopulorum. Vulnerability curves showing loss of conductivity (...

OBJECTIVES To investigate the viability and differentiation capacity of dental pulp stem cells (DPSCs) isolated from single donors after 2 years of cryopreservation.   METHODS This prospective study was conducted between October 2010 and February 2014 in the Stem Unit, College of Medicine, King Saud University, Riyadh, Saudi Arabia. Seventeen teeth extracted from 11 participants were processe...

Recent fire activity throughout Alaska has increased the need to understand postfire impacts on soils and permafrost vulnerability. Our study utilized data and modeling from a permafrost and ecosystem gradient to develop a mechanistic understanding of the shortand long-term impacts of tundra and boreal forest fires on soil thermal dynamics. Fires influenced a variety of factors that altered the...

Permafrost soils store over half of global soil carbon (C), and northern frozen peatlands store about 10% of global permafrost C. With thaw, inundation of high latitude lowland peatlands typically increases the surface-atmosphere flux of methane (CH4), a potent greenhouse gas. To examine the effects of lowland permafrost thaw over millennial timescales, we measured carbon dioxide (CO2) and CH4 ...

In the present study, motility, ultrastructure and fertilization capacity of fresh and cryopreserved striped bass spermatozoa were investigated in order to evaluate semen dilution ratio, freezing rate and cryomedia. Four dilution ratios (semen/cryomedia), and four freezing rates were evaluated on the basis of post-thaw sperm motility. The dilution ratio of 1:3 yielded the highest (P < 0.05) pos...

The mucoadhesive hydrogel film was prepared and optimized for the purpose of local drug delivery to oral cavity for the treatment of oral Candidiasis. The mucoadhesive hydrogel film was prepared with the poly(vinyl alcohol) by freeze/thaw crosslinking technique. 3(2) full factorial design was employed to optimize the formulation. Number of freeze/thaw cycles (4, 6, and 8 cycles) and the concent...

OBJECTIVE To determine the clinical potential of donor-oocyte cryopreservation and thaw techniques for recipient patients. DESIGN Institutional review board-approved prospective study of donor oocyte cryopreservation. SETTING A large, private infertility center. PATIENT(S) Four anonymous oocyte donors underwent ovarian hyperstimulation for the purpose of oocyte retrieval and cryopreservat...