Single-fluorescent protein biosensors (SFPBs) are an important class of probes that enable the single-cell quantification of analytes in vivo. Despite advantages over other detection technologies, their use has been limited by the inherent challenges of their construction. Specifically, the rational design of green fluorescent protein (GFP) insertion into a ligand-binding domain, generating the...

The present article describes the TSC Protocol (tubes, support, connectors) version 2.2 for construction of surgical guides implant placement and possibility insertion implants also.

This paper presents an algorithm for interconnect layout optimization with bu er insertion. Given a source and n sinks of a signal net, with given positions and a required arrival time associated with each sink, the algorithm nds a bu ered Steiner tree so that the required arrival time (or timing slack) at the source is maximized. In the algorithm, Steiner routing tree construction and bu er in...

Third-generation synchrotron light sources are specifically designed to operate with long insertion devices that produce very high brightness beams of synchrotron radiation. There are many such facilities now under construction, or in the design stage, allover the world. After a brief review of the main properties of the low emittance storage rings that form the heart of these facilities, we wi...

Background: Vulvovaginal Candidiasis is one of the most common vaginal infections and identification of candida species can be helpful in medical treatment. Intra uterine devices (IUDs) are effective, long-term methods of contraception, which in some cases may lead to Vulvovaginal Candidiasis. This study was developed to assess the effect of IUD insertion on Candida fungal flora in cervicovagin...

Transmission loss and insertion loss measurements of building materials at audible frequencies are commonly made using plane wave tubes or as a panel between reverberant rooms. These measurements provide information for noise isolation control in architectural acoustics and in product development. Airborne ultrasonic sound transmission through common building materials has not been fully explor...

Background: There is some demand for nasogastric tube insertion in unconscious or ICU patients. Nasogastric tubes are generally made of flexible plastic materials, prone to twisting and deviation by the tracheal tube, when passing through the pharynx and esophageal opening, making it difficult to insert the NG-tube properly. We hypothesized that NG-tube insertion with help of guidewire can sign...

aim and background:   safe airway maintenance in patients under general anesthesia is the most important duty of anaesthesiologists. classic laryngeal mask airway (clma) is a supra-glottic device to achieve this aim. there are different insertion techniques for this device, but the best of them is unknown. methods and materials:   two hundred patients were included in this mono-blinded clinical...

As an introduction, I want to contrast two general traditions in semantics, one realistic and one cognitive. According to the realistic approach to semantics the meaning of an expression is something out there in the world. In technical terms, a semantics for a language is defined as a mapping from the syntactic structures to things in the world. In philosophical ontological terms, this is thus...