Exposure to nanoparticles (NPs) may cause vascular effects including endothelial dysfunction and foam cell formation, with oxidative stress and inflammation as supposed central mechanisms. We investigated oxidative stress, endothelial dysfunction and lipid accumulation caused by nano-sized carbon black (CB) exposure in cultured human umbilical vein endothelial cells (HUVECs), THP-1 (monocytes) ...

BACKGROUND Curcumin is a principal compound of turmeric, commonly used to treat tumors and other diseases. However, its anti-cancer activity in human acute monocytic leukemia THP-1 cells is not clear. This study aimed to study the anti-cancer effect and action of curcumin on THP-1 cells. METHODS THP-1 parental cells and PMA-treated THP-1 cells, were used as in vitro models to evaluate the ant...

BACKGROUND Human colon contains many bacteria that commonly colonize the perineum and frequently enter the urinary tract. Uropathogenic Escherichia coli are the most common cause of urinary tract infection. Type 1 fimbriated E. coli have been associated with cystitis, and P fimbriated E. coli with pyelonephritis. Factors involved in clearing bacteria from the urinary tract are poorly understood...

Circulating complexes of leukocytes and activated platelets are markers for atherosclerosis, but their interaction with the arterial endothelial lining has not been studied. Therefore, the effect of activated platelets on rolling and adhesion of labeled human THP-1 monocytoid cells to human umbilical vein endothelial cell (HUVEC) monolayers was studied by epifluorescence microscopy in a paralle...

The identification and characterization of cell surface markers present on different subtypes of cells of the immune system can broaden the understanding of immune cell function. The BD LyoplateTM human cell surface marker screening panel is a collection of cell surface marker antibodies designed for screening cells for the expression of 242 human cell surface proteins by flow cytometry or fluo...

A human monocyte-derived cell line (THP-1) was used as a model to investigate the role of metallothionein (MT) in the cellular physiology of resting and activated monocytes. MT protein levels were reduced in THP-1 cells by transient transfections with an antisense MT expression vector. Antisense mouse MT-1 RNA was constitutively expressed under the control of the H-2Kb (mouse major histocompati...

Cardiovascular diseases are the leading causes of death worldwide, closely followed by cancer. To investigate impact breast cancer cell lines (SKBR3, MCF-7, and MDA-MB-231) on endothelial adhesion, a blended medium containing 30% breast-cancer-conditioned was prepared. This then exposed to human umbilical vein cells (HUVECs) monocytes (THP-1) for 48 h. Homemade oxidized low-density lipoproteins...

Macrophages are an important part of the cellular immune system and play a key role during immune responses. Thus, macrophages are interesting targets in basic and clinical research. Primary monocytes or monocyte-derived macrophages do not proliferate on a suitable scale so that their use for functional studies in vitro is limited. Immortal proliferating cell lines, such as the human THP-1 mono...