In this automated apoenzyme reactivation immunoassay system (Ames Optimate’”)forthyroxin-binding globulin (TBG),thesampleand M-aminohexylflavin adeninedinucleotide-labeled TBG reactsequentially withantiserum. Then apoglucose oxidaseis added to combine with the free fraction and generate glucose oxidase activity, which is measured colorimetrically. The assay requires 100 .tLof sample and covers ...

is between 0.40 and 0.70 mmol/L. Granted, our patients are type I rather than type U diabetics, but both are allegedly prone to have increased concentrations of free glycerol in plasma. 3. We serve as the clinical laboratory for the University of Minnesota's hyperlipidemia clinic and I see patients in that clinic. This clinic's patient population is principally referral patients with more sever...

In binding assays for determination of free (non-protein-bound) hormones in serum or plasma, the influence of the measuring system on the original analyte concentration in the sample must be considered. In one- or single-step free-hormone immunoassays, the labeled analyte or analog-tracer not only is bound to the antibody, it also is bound, to some extent, to serum proteins. The dependence of t...

When concentrations of free thyroid hormones in serum are measured by equilibrium dialysis, the serum sample is diluted by dialysis buffer, resulting in a new equilibrium for distribution of these hormones that differs from that in the original serum sample. We have derived a mathematical formulation, based on an equilibrium model for multiple ligands and multiple binding sites, to determine th...

In 18 cases of serious and fairly serious toxemic pregnancy, the authors gave 500 micrograms L-Thyroxin "Henning" intraamniotically after the laboratory evaluation demonstrated negative pulmonary maturity from an amniotic fluid sample. Maternal and fetal complications due to the drug could not be observed. Despite a 39% premature incidence, IRDS and hyaline membrane disease were not observed. T...

The aim of the present work was to determine whether the increase in monoamine oxidase activity previously found in hyperand hypothyroid rats, was due to thyroxin effects on enzymatic activity or to variations in enzyme synthesis. The rate of synthesis of monoamine oxidase in young hyperand hypothyroid rats has been studied by measuring the recovery of enzymatic activity 7 days after irreversib...

We have designed a re-usable dialysis cell and a complex dialysis buffer, with which undiluted serum samples can be dialyzed with minimal changes in their serum matrix. Dialysate thyroxin (free T4) is then measured by a sensitive RIA for T4. The range of reportability was 2-128 ng/L, the normal range was 8-27 ng/L, and the interassay CV was 7%. Free T4 concentrations in various disorders were a...