The aim of the present study was to determine seroprevalence of Toxoplasma gondii infectionin stray cats and correlation with oocyst shedding and IFN-γ concentration. From April to August 2016, one hundred fifty-nine stray cats were captured from various localities in Mashhad area. The blood and fecal samples were collected from each cat. The serum samples were examined to detect antib...

Toxoplasma gondii is a common protozoan parasite that infects warm-blooded animals throughout the world, including mice and humans. During infection, both, the parasite and the host, utilize various mechanisms to maximize their own reproductive success. Mice and humans are both the intermediate hosts for Toxoplasma gondii, which forms specialized vacuoles containing reproductive cysts in the fo...

Objective: Sera from 186 sheep (93 rams and 93ewes) from Tabriz, North-West of Iran were evaluated for antibodies to Toxoplasma gondii using a commercial ELISA kit. Methods: The animals were divided into two age groups (< 2 years, and > 2 years). Results: Antibodies to T. gondii were found in 13 of 186 sheep (6.98%). Infection rate in ewes was higher than rams but this differe...

Toxoplasmosis caused by Toxoplasma gondii is an asymptomatic disease, but often takes a severe and life-threatening course during pregnancy, in foetuses, new bon babies immune-compromised people.

OBJECTIVE Our aim was to determine the Toxoplasma gondii IgG seroprevalence in HIV/AIDS patients who applied to our outpatient clinic. METHODS Between January 2006 and June 2010, 164 HIV/AIDS patients were tested for Toxoplasma gondii IgG antibodies by using the ELISA method. RESULTS Of the total of 164 HIV/AIDS patients, 135 were male, 29 were female with a mean age of 36 years (range: 20-...

Intramuscular administration of Toxoplasma gondii lysate antigens to mice produced titers of T. gondii-specific antibody (measured by Sabin-Feldman dye test) greater than or equal to 1:1,024 in their sera. Intravenous administration of heat-killed Propionibacterium acnes to mice produced peritoneal macrophages with enhanced microbicidal capacity against T. gondii. Mice with high antibody titers...

To study the distribution of tissue cysts in porcine tissues, 16 pigs were fed oocysts of 4 strains of Toxoplasma gondii (4 pigs/strain). Pigs were euthanatized between postinoculation days 103 and 875 and portions of 5 to 14 organs were bioassayed in mice and/or cats for T gondii. For bioassays, 50- to 100-g portions of tissue were incubated in acidic pepsin solution to free bradyzoites from c...

Toxoplasmosis is a common and widespread infection in humans and many other species of warm–blooded animals. Toxoplasma gondii-derived heat shock protein 70 (Hsp70) may play an important role in the virulence of Toxoplasma gondii (T. gondii). In the present study, T. gondii Hsp70 was amplified by polymerase chain reaction (PCR) from the DNA of the T. gondiitachyzoite RH strain through the use o...

ONCEL, T., G. VURAL: Occurrence of Toxoplasma gondii antibodies in sheep in Istanbul, Turkey. Vet. arhiv 76, 547-553, 2006. ABSTRACT This study was carried out in order to determine the occurrence of Toxoplasma gondii antibodies in sheep in five districts of Istanbul, Turkey. A total of 181 sera taken from sheep were examined for antibodies to Toxoplasma gondii using an enzyme-linked immunosorb...