Thrombopoietin (TPO) has been used in preclinical myelosuppression models to evaluate the effect on hematopoietic reconstitution. Here we report the importance of dose and dose scheduling for multilineage reconstitution after myelosuppressive total body irradiation (TBI) in mice. After 6 Gy TBI, a dose of 0.3 microgram TPO/mouse (12 microgram/kg) intraperitoneally (IP), 0 to 4 hours after TBI, ...

• TPO specifically activates Erk and NF-kB pathways in hematopoietic stem and progenitor cells. • Erk and NF-kB cooperate to trigger their common target, Iex-1, and DNA-PKdependent NHEJ activation in HSPCs upon irradiation. Loss of hematopoietic stem cell (HSC) function and increased risk of developing hematopoietic malignancies are severe and concerning complications of anticancer radiotherapy...

Objectives: To find an association between circulating oestradiol and thyroid autoimmunity in females with polycystic ovarian syndrome (PCOS) its impact on their health. Materials Methods: This study was conducted at Aziz Fatimah Medical Dental College, after obtaining ethical approval from Institutional Ethical Committee (letter No. DME/568-19) April to September 2017. Hundred PCOS enrolled th...

In moderately iodine-deficient, pregnant, thyroid peroxidase antibody (TPO-Ab)-positive women the role of iodine supplementation in the development of postpartum thyroid dysfunction (PPTD) was studied in a placebo-controlled, randomized, double blind trial. Screening for TPO-Ab was performed in early pregnancy in a population of healthy pregnant Danish women with no previous diagnosed thyroid d...

We have studied the effects of recombinant human thrombopoietin (TPO, Mpl ligand) on human hematopoiesis in vitro. TPO alone did not support erythroid burst formation but. in the presence of erythropoietin. it enhanced erythroid burst formation from CD34+ bone marrow and cord blood cells. The burst-promoting activity (BPA) was stronger under 5% serum than 30% serum conditions. The direct nature...

BACKGROUND AND PURPOSE Thrombopoietin (TPO), a growth factor primarily involved in thrombopoiesis may also have a role in the pathophysiology of sepsis. In patients with sepsis, indeed, TPO levels are markedly increased, with disease severity being the major independent determinant of TPO concentrations. Moreover, TPO increases and correlates with ex vivo indices of platelet activation in patie...

The four-ball tester was used in this analysis to demonstrate the lubricity of tire pyrolysis oil (TPO). tribological performance compared with diesel fuel (DF) and their blends, DT10 (TPO 10%, Diesel 90%) DT20 20%, 80%). A scanning electron microscope (SEM) investigate wear scar. In contrast fuel, TPO demonstrated better antiwear behaviour terms higher load-carrying capacity. DT10, DT20, TPO’s...

Identification and enumeration of human hematopoietic stem cells remain problematic, since in vitro and in vivo stem cell assays have different outcomes. We determined if the altered expression of adhesion molecules during stem cell expansion could be a reason for the discrepancy. CD34+CD38- and CD34+CD38+ cells from umbilical cord blood were analyzed before and after culture with thrombopoieti...