Cell transfection is an essential technique for interrogating cellular pathways. Determining the optimal conditions for introducing nucleic acids into cells can be a time consuming endeavor at the initiation of a cell biology experiment or screen. Here we demonstrate how the Biomek FXP Workstation can be used to identify the optimal conditions for siRNA transfection in a single experiment. Util...

Macrophage migration inhibitory factor (MIF) is closely associated with tumorigenesis. The present study aimed to investigate the effects of MIF on the proliferation, migration and colony formation of oral squamous cell carcinoma (OSCC), and to quantify the protein expression levels of MIF in OSCC tissue samples. Firstly, small interfering (si) RNA was used to knock down the gene expression of ...

The introduction of nucleic acids into cells is one of the most valuable and frequently used tools of biological science. Transfection methods are used for a range of applications, including gene function studies, modulation of gene expression, biochemical mapping, mutational analysis and production of recombinant proteins. The transfection techniques which are commonly used today can be classi...

Nonviral gene delivery methods are advantageous over viral vectors in terms of safety, cost, and flexibility in design and application, but suffer from lower gene transfer efficiency. In addition to modifications to nucleic acid design and nonviral carriers, new tools are sought to enhance transfection. Priming is the pharmacological modulation of transfection efficiency and transgene expressio...

Site-directed RNA editing (SDRE) technologies have great potential in gene therapy. Our group has developed a strategy to redirect exogenous adenosine deaminases acting on (ADARs) specific sites by making editable structures using antisense oligonucleotides. Improving the efficiency of MS2-ADAR system is important treating undesirable G-to-A point mutations. This work demonstrates an effective ...

The protein corona can significantly modulate the physicochemical properties and gene delivery of polyethylenimine (PEI)/DNA complexes (polyplexes). effects on transfection have been well studied in terms averaged expression a whole cell population. Such evaluation methods give excellent reliable statistics, but they general provide final efficiency without reflecting dynamic process expression...

Calcium phosphate nanoparticles (CaP-NP) are ideal tools for transfection due to their high biocompatibility and easy biodegradability. After transfection these particles dissociate into calcium and phosphate ions, i.e. physiological components found in every cell, and it has been shown that the small increase in intracellular calcium level does not affect cell viability. CaP-NP functionalized ...

toxoplasmosis is a worldwide infection which is commonly asymptomatic but can cause serious medical problems in immunocompromised individuals and fetus. the infection also causes considerable economic loss because of abortion in livestock, mostly in sheep and goats. dna vaccination may be a powerful approach against intracellular parasites such as toxoplasma gondii. the goal of this study was t...

Introduction: Multiple sclerosis (MS) is a disease of the immune system: it attacks the myelin around the axons and leaves them exposed. Destruction of myelin weakens the electrical conduction of ions and thus leads to a lack of communication in the nervous system.  Methods: In the present study, we constructed recombinant plasmid and then transformed to E. coli cell. The colonies containing...