manipulation of different genes in crop plants to get desirable characters has become an important tool of plant biotechnology. in the current study, cotton variety mnh-786 was modified for its characteristics to show resistance against lepidopteran insects and herbicide by transformation of cry1ac+cry2a and gtgene cloned in a different cassette under 35s promoter. mature embryos of cotton mnh-...

Maize and wheat are some of the world's most important food crops, so their breeding programs for global security. Genome editing techniques (the latest advancement in genetics) not replacements conventional techniques, they new methods innovative, which promote agricultural crop offers unprecedented solutions to insecurity. Among these CRISPR-Cas9 is considered a more effective tool genome due...

In reference to what was published today at Scidev (Gould, 2006): “GM crops are compatible with sustainable agriculture”, I wish to comment: Massive use of transgenic crops poses potential ecological risks; genetically modified (GM) crops are not compatible with sustainable or organic farming or any other alternative form of production. GM agriculture compromises the capacity of farmers to mana...

Retrospective and prospective studies show that lean mass or strength is positively associated with metabolic health. Mice deficient in myostatin, a growth factor that negatively regulates skeletal muscle mass, have increased muscle and body weights and are resistant to diet-induced obesity. Their leanness is often attributed to higher energy expenditure in the face of normal food intake. Howev...

UNLABELLED While cervical cancer incidence and mortality rates have declined in the United States, this cancer represents a worldwide threat. Human papilloma viral infection causes cervical neoplasia (CIN). 3,3'-Diindolylmethane (DIM) prevents or inhibits the progression from cervical dysplasia to cancer. The aim of this study is to determine the most effective dose of DIM given continuously in...

Activation of melanocortin-4-receptors (MC4Rs) reduces body fat stores by decreasing food intake and increasing energy expenditure. MC4Rs are expressed in multiple CNS sites, any number of which could mediate these effects. To identify the functionally relevant sites of MC4R expression, we generated a loxP-modified, null Mc4r allele (loxTB Mc4r) that can be reactivated by Cre-recombinase. Mice ...

The main aim of this study was to obtain the Crimean-Congo hemorrhagic fever virus (CCHFV) glycoprotein, through either stable transgenic plants or using a transient expression system, and determine the yield, quality and finally the immunogenicity of the plant-made CCHFV glycoprotein in a mouse model. We designed and synthesized a codon-optimized G1/G2 gene from the G1 and G2 parts of the CCHF...

A significant portion of the world’s cultivated land is affected by salinity that reduces crop productivity in these areas. Breeding for salt tolerance is one of the important strategies to overcome this problem. Recently, genetic engineering is becoming a promising approach to improving salt tolerance. In order to improve the yield performance of canola in saline soils, we transformed canola w...