Stably transformed Arabidopsis lines in which GUS marked cell clones are readily produced in response to heat-shock have been established and characterized. Control of GUS activation is achieved by heat-shock-induced FLP recombinase activity which "switches on" expression of a GUS marker gene previously held transcriptionally silent. To obtain efficient GUS sectoring, single insert Arabidopsis ...

The expression of the jellyfish green fluorescent protein (GFP) in plants was analyzed by transient expression in protoplasts from Nicotiana tabacum, Arabidopsis thaliana, Hordeum vulgare, and Zea mays. Expression of GFP was only observed with a mutated cDNA, from which a recently described cryptic splice site had been removed. However, detectable levels of green fluorescence were only emitted ...

The arc,4 , a member of the G protei" ll-sllbunit fHmi]y, was isolated from tobacco BY-2 cells as an auxin-responsive gene. Characterization of arc.4, which should help to elucidate the function of the gene product in the plant cells, was performed with emphasis on the mode of expression and the analysis of its promoter. Accumulation of the arct4 message was detected only after treatments with ...

• An effective transformation method is described for Medicago truncatula A17, verifying its suitability as a model legume for functional genomics. • Media and culture methods are detailed that yielded an average frequency of 35% for recovery of transgenic shoots from cotyledonary node explants and 39% for root induction and regeneration of entire plants from 419 phosphinothricinresistant shoot...

Expression of the PrfA-controlled virulence gene hly (encoding the pore-forming cytolysin listeriolysin) is under negative regulation by readily metabolized carbon sources in Listeria monocytogenes. However, the hyperhemolytic strain NCTC 7973 exhibits deregulated hly expression in the presence of repressing sugars, raising the possibility that a defect in carbon source regulation is responsibl...

Agrobacterium tumefaciens strain EHA 105 carrying binary vector pCAMBIA 1301 was used for transformation in two economically important highly recalcitrant indica rice cultivars HKR-46 and HKR126. High concentrations of acetosyringone in the Agrobacterium culture and co-cultivation medium proved to be indispensable for successful transformation. Embryogenic scutellar calli were used for transfor...

conclusions by employment of gene optimization strategies, use of viral-based vectors and suppression of plant-derived gene silencing effect, efficient transient expression of hcvcp in tobacco with proper antigenic properties could be possible. background hepatitis c virus (hcv) is major cause of liver cirrhosis in humans. hcv capsid (core) protein (hcvcp) is a highly demanded antigen for vario...

DNA elements involved in the regulation of two sunflower helianthinin genes were identified by analysis of beta-glucuronidase (GUS) expression in transgenic tobacco driven by sequences derived from the 5' upstream regions of these genes. A 2.4-kb upstream region of the helianthinin gene HaG3-A conferred rigorous developmental GUS expression in transgenic tobacco seeds with no significant GUS ac...

In one of 30 transgenic tobacco (Nicotiana tabacum) plants, the expression of an introduced beta-glucuronidase (GUS) gene driven by the cauliflower mosaic virus 35S promoter, was found to be repressed as the plant matured, whereas the endogenous GUS activity was unaffected. Plants grown from seeds or regenerated from leaf discs derived from this plant showed a similar temporal pattern of expres...

Genes encoding phenyla]anine ammonia-tyase (PAL) form a small multigene family with at least three members in pea. Tissue-specific expression of the promoter of a member of PAL gene family (RSR4Ll) was inyestigated in the transgenic tobacco transformants earrying the different modes of chimeric fusion between the RSItaLl promoter and a bacteria] ll-glucuronidase (GUS) gene. In stems, at least, ...