BACKGROUND While DNA barcoding is an important technology for the authentication of the botanical origins of Chinese medicines, the suitable markers for DNA barcoding of the genus Uncaria have not been reported yet. This study aims to determine suitable markers for DNA barcoding of the genus Uncaria (Gouteng). METHODS Genomic DNA was extracted from the freshly dried leaves of Uncaria plants b...

Paphiopedilum là một chi nổi bật của nhóm lan Hài, đây nhỏ nhưng có hệ thống phân loại phức tạp với nhiều quan điểm khác nhau. canhii phát hiện năm 2010 và được coi loài đặc hữu Việt Nam. Các nghiên cứu về cho thấy vị trí rất riêng biệt chúng. Để chính xác này, người ta đã phải sử dụng tiêu chí kết hợp. Hiện nay, ngoài phương pháp truyền bằng hình thái, thì chỉ thị tử triển thể ưu khi các học. ...

DNA barcoding is expected to be one of the most promising tools in biological taxonomy. However, there have been no agreements on which core barcode should be used in plants, especially in species-rich genera with wide geographical distributions. To evaluate their discriminatory power in large genera, four of the most widely used DNA barcodes, including three plastid regions (matK, rbcL, trnH-p...

The current tribal classification of Myrtaceae was based on analysis the plastid matK coding region within trnK intron. phylogenetic position genera Cloezia and Xanthomyrtus poorly supported, original sequence for Kania, type genus tribe Kanieae, rather poor. To clarify relationships, we sequenced psbA–trnH an extended portion intron, including spacer regions flanking matK, nuclear ribosomal IT...

Lauraceae are an important component of tropical and subtropical forests and have major ecological and economic significance. Owing to lack of clear-cut morphological differences between genera and species, this family is an ideal case for testing the efficacy of DNA barcoding in the identification and discrimination of species and genera. In this study, we evaluated five widely recommended pla...

Biological invasions are regarded as threats to global biodiversity. Among invasive aliens, a number of plant species belonging to the genera Myriophyllum, Ludwigia and Cabomba, and to the Hydrocharitaceae family pose a particular ecological threat to water bodies. Therefore, one would try to prevent them from entering a country. However, many related species are commercially traded, and distin...

Piper species are used for spices, in traditional and processed forms of medicines, in cosmetic compounds, in cultural activities and insecticides. Here barcode analysis was performed for identification of plant parts, young plants and modified forms of plants. Thirty-six Piper species were collected and the three barcode regions, matK, rbcL and psbA-trnH spacer, were amplified, sequenced and a...

It is long believed that some spices may help protect against certain chronic conditions. Spices are usually parts of plants that have been powdered into small pieces. Have you ever wondered what the curry powder in your dish is made of? The aim of this work was to develop an appropriate DNA-based method for assessment of spice identity. Selecting the best marker for species recognition in the ...

Recent studies indicate that the discriminatory power of the core DNA barcodes (rbcLa + matK) for land plants may have been overestimated since their performance have been tested only on few closely related species. In this study we focused mainly on how the addition of complementary barcodes (nrITS and trnH-psbA) to the core barcodes will affect the performance of the core barcodes in discrimi...