Single-chain urokinase (SC-UK) has an intrinsic amidolytic activity. as measured with synthetic substrate (Kabi 52444; pyro-GIu-GIy-Arg-pNitroanalide). which was found to be 0.1 % to 0.2% that of its plasmin-activated derivative, two-chain UK (TC-UK). A study of the reaction of SC-UK with plasminogen is complicated by the effect of the reaction product, plasmin, on both reactants. The resultant...

Thrombin hydrolyzes the Arg156-Phe157 bond in pro-urokinase (pro-UK), two residues from the activation site, generating a two-chain form (thromb-UK) believed to have little activity and that is resistant to plasmin activation. The kinetic constants for thromb-UK against synthetic substrate (S2444) were found to be essentially identical to pro-UK. Against native plasminogen, thromb-UK had a lowe...

1 Nuffield Institute for Health, University of Leeds, UK 2 Centre for Health Economics, University of York, UK 3 NHS Centre for Reviews and Dissemination, University of York, UK 4 BHF Heart Research Centre at Leeds, Leeds General Infirmary, UK 5 Clinical Trials and Evaluation Unit, Royal Brompton and Harefield NHS Trust, London, UK 6 Division of Public Health Sciences, University of Nottingham,...

M Adams Acoustics Research Centre, University of Salford, Salford, UK N Bruce Acoustics Research Centre, University of Salford, Salford, UK W Davies Acoustics Research Centre, University of Salford, Salford, UK R Cain WMG, University of Warwick, UK P Jennings WMG, University of Warwick, UK A Carlyle London College of Communication, University of the Arts, UK P Cusack London College of Communica...

1Department of Endocrinology, University College Hospital, London, UK 2National Hospital for Neurology and Neurosurgery, London, UK 3Physicians’ Clinic, London, UK 4Department of Endocrinology, St Bartholomew’s Hospital, London, UK 5Endocrinology/General Medicine, University Hospitals Coventry and Warwickshire NHS Trust, University of Warwick, Coventry, UK 6The Lister Hospital, London, UK 7Roya...

1 Nuffield Department of Anaesthetics, Oxford University Hospitals NHS Trust, Oxford, UK 2 Department of Psychology, School of Psychology and Cognition Institute, Plymouth University, Plymouth, UK 3 Department of Anaesthesia, Nottingham University Hospitals NHS Trust, Nottingham, UK 4 Member Royal College of Anaesthetists’ Lay Committee, London, UK 5 Department of Anaesthesia, Intensive Care an...

MRC Unit for Lifelong Health and Ageing, Research Department of Epidemiology and Public Health, University College London, London, UK, Clinical Radiology, Manchester Royal Infirmary, Oxford Road, Manchester, UK, Vascular Physiology Unit, Institute of Child Health, University College London, London, UK, MRC Epidemiology Unit, Cambridge, UK, Cardiovacular Institute, Sahlgrenska University Hospita...

H. Qureshi,1 E. Massey,2 D. Kirwan,3 T. Davies,4 S. Robson,5 J. White,6 J. Jones7 & S. Allard8 1Department of Haematology, University Hospitals of Leicester, Leicester, UK, 2NHS Blood & Transplant, Bristol, UK, 3NHS Fetal Anomaly Screening Programme, UK National Screening Committee, University of Exeter, Exeter, UK, 4NHS Blood & Transplant, Manchester, UK, 5Department of Fetal Medicine, Institu...

Worcestershire Royal Hospital, Worcestershire Acute Hospitals NHS Trust, Worcester, UK The University of Manchester, Manchester, UK The University of Manchester, Manchester Academic Health Science Centre, University Hospital South Manchester NHS Foundation Trust, Manchester, UK University Hospital of South Manchester, Manchester, UK Royal Blackburn Hospital, Lancashire, UK Lane Fox Unit, St Tho...

Worcestershire Royal Hospital, Worcestershire Acute Hospitals NHS Trust, Worcester, UK The University of Manchester, Manchester, UK The University of Manchester, Manchester Academic Health Science Centre, University Hospital South Manchester NHS Foundation Trust, Manchester, UK University Hospital of South Manchester, Manchester, UK Royal Blackburn Hospital, Lancashire, UK Lane Fox Unit, St Tho...