A technique for the preparation of thyroglobulin by fractional precipitation with either ammonium sulfate or potassium phosphate buffer was described in 1948 by Derrien et al. (1). These preparations were found to be homogeneous by boundary electrophoresis (l), but heterogeneous in their salting-out curves (1) and in the ultracentrifuge (2, 3). In the ultracentrifuge, a main component with a se...

BACKGROUND The development of antibodies to thyroid peroxidase, thyroglobulin, and thyroid-stimulating hormone (TSH) receptor is a main feature of autoimmune thyroid diseases. OBJECTIVE To investigate whether complete removal of thyroid antigens results in the abatement of humoral thyroid autoimmunity. DESIGN Retrospective chart review study of patients treated and monitored with a standard...

In each of three separate experiments, female guinea pigs in groups of 20 were given 4 units of thyroid-stimulating hormone (TSH) each day for 3 days, while controls were given saline. Na125I was injected on the 3rd day, and the animals were killed 22 hours later. The pooled throids of each group were homogenized, and thyroglobulin was purified by one of the following methods: gel filtration on...

OBJECTIVE Thyroid hormones influence the GH/IGF1 axis, but previous studies have reported discrepant results regarding serum IGF1 levels in hyperthyroidism. We have therefore investigated, at diagnosis, the relationship between serum IGF1 levels and the main characteristics of Graves' disease (GD): severity of hyperthyroidism, goiter size, presence of active Graves' ophthalmopathy (GO), antythy...

background: infections attributed to helicobacter pylori ( h. pylori ) involve almost half of the world's population. one of the effects is auto-antibody induction and cross-reaction with numerous proteins in the body. as a result of its widespread prevalence and importance, this study evaluates the associations between h. pylori and thyroid auto-antibodies. materials and methods: this study en...