The involvement of phospholipase A2 (PLA2) and fatty acid release in the regulation of sodium-dependent high-affinity choline uptake in rat brain was assessed in vitro through the use of the specific binding of 3H-hemicholinium-3 (3H-HCh-3). Addition of arachidonic acid and other unsaturated fatty acids to rat striatal membranes in vitro resulted in a dose-dependent, temperature-independent act...

Both cis- and trans-unsaturated but not saturated fatty acids activated protein kinase C purified to apparent homogeneity from rat brain. Fatty-acid-induced enzyme activation was not more than additive with that by phospholipids and was potentiated by diacylglycerol. Recently, we demonstrated that cis- and trans-unsaturated fatty acids induced platelet aggregation and phosphorylation of specifi...

The objective of this study was to evaluate the effects of halogenated compounds, organic acids, unsaturated fatty acids and their mixtures on in vitro methane production and fermentative characteristics of mixed rumen microorganisms. Agents used in two in vitro experiments were bromoethanesulfonic acid (BES) and pyromellitic diimide (PMDI) as halogenated compound, fumarate and malate as organi...

Barotolerant bacterium was isolated from sediment sample which was obtained from the depth of 4033 m in the Izu-Ogasawara Trench. The physiological property, growth characteristics and fatty acid composition were examined. The strain was a psychrotrophic and barotolerant bacterium, and was identified as species in the genus Alteromonas. The fatty acids of the strain were from C12 to C18. As the...

The effect of fatty acids with various structure and length in the hydrocarbon chain on the purified AMP deaminase from bovine brain was investigated. All the saturated fatty acids and unsaturated ones of trans configuration, longer than 16 in the hydrocarbon chain, were activators of the enzyme, while the unsaturated fatty acids of cis configuration including oleic, linoleic, linolenic, arachi...

In this study rainbow trout (Oncorhynchus mykiss) fillets were vacuum packed, frozen and stored at -18°C and  the effect of this packaging method on proximate composition, fatty acids and amino acid profile and their changes compared to control conditions during 9 months of storage were studied. The results showed that 18 fatty acids were identified. The unsaturated fatty acid (UFA) were 56.77 ...

Eight antifungal agents were examined for effects on lipid biosynthesis and membrane integrity in Candida albicans. Lipids were labeled in vivo or in vitro with [14C]acetate and analyzed by thin-layer and gas chromatography. Membrane integrity was measured by a recently developed [14C]aminoisobutyric acid radiolabel release assay. The imidazole antifungal agents miconazole, econazole, clotrimaz...

When Streptococcus salivarius was grown in batch culture in the presence of various Tween detergents, the fatty acid moiety of the detergent was incorporated into the lipids of its membrane. Tween 80 (containing primarily oleic acid) markedly stimulated the production of extracellular glucosyltransferase and also increased the degree of unsaturation of the membrane lipid fatty acids. The possib...

changes in the fatty acid composition and biochemical indices of mackerel (which has a substantial lipid content) and shark (which has negligible lipid content) fillets stored at - 18°c for up to six months were measured. lipid content was measured (6.35% and 1.38%) in mackerel and shark, respectively; however it decreased during frozen storage in both fish species. in analysis of fatty acids t...

Investigations of recalcitrant, or desiccation-sensitive, seeds have as yet failed to identify the causes of this phenomenon. Experiments with Guarea guidonia (L.) Sleumer (American muskwood) were initiated to determine the effects of desiccation on the physiology and biochemistry of the seeds of this tropical tree species. Seeds were air-dried at room temperature for 7 days. At intervals, germ...