The safety and shelf-life of food products can be, respectively, ensured and extended with important food-processing technologies such as irradiation. The joint effect of cooking and 10 kGy gamma irradiation on the fatty acid composition of the oil of Pentaclethra macrophylla Benth was evaluated. Oils from the raw seed, cooked seeds, irradiated seeds (10 kGy), cooked, and irradiated seeds (10 k...

A procedure is described for selection of temperature-sensitive mutants affecting fatty-acid synthesis based upon radiation suicide of wild-type organisms by tritiated acetate selectively incorporated into fatty acids. At 37 degrees , two of the mutants extensively incorporate fatty-acid supplements provided in the medium, and grow for extended periods only when a trans-unsaturated or a combina...

The technique of gas-liquid chromatography-chemical ionization mass spectrometry can easily identify trace peaks and unresolved peaks on gas-liquid chromatography, utilizing MH(+) ions of chemical ionization mass spectra. In polyunsaturated fatty acid methyl esters such as C22:5 and C22:6, the determination of molecular weights that are difficult to determine by electron impact mass spectrometr...

Fatty acid composition of beef intramuscular tissue is an important trait because high proportions of mono-unsaturated fatty acid are related to favorable beef flavor. In this study, we investigated the effects of genetic factors, such as stearoyl-CoA desaturase (SCD), on beef carcass traits, including fatty acid composition, in the Hanwoo. Analysis of fatty acids in Hanwoo was performed using ...

The saturated fatty acid auxotroph of Saccharomyces cerevisiae, KD115, was used to determine the efficiency of various unsaturated fatty acids in supporting growth. The efficiency, as the number of cells produced per fmol of unsaturated fatty acid, ranged from zero for a number of acids to over 26 cells per fmol of eicosapentaenoic acid. Efficiencies tended to be higher for acids with fewer car...

Unsaturated fatty acids are important constituents of all cell membranes and are required for normal growth. In the filamentous fungus Aspergillus nidulans, unsaturated fatty acids and their derivatives also influence asexual (conidial) and sexual (ascospore) sporulation processes. To investigate the relationship between fatty acid metabolism and fungal development, we disrupted the A. nidulans...

The breakdown of cellular components via autophagy is crucial for cellular homeostasis.In this issue of The EMBO Journal,Niso-Santano et al (2015) report the important observation that feeding cells with saturated or unsaturated fatty acids triggers mechanistically distinct autophagic responses. Feeding cells saturated fatty acid induced the canonical, BECN1/PI3K dependent autophagy pathway. Co...

Wild fruits are commonly consumed in the rural communities of South Africa. The information on their nutritionally important fatty acids is, however, limited. Three wild fruit species, Diospyros blancoi, Landolphia kirkii and Sclerocarya birrea from Limpopo Province were selected for evaluation of fatty acid content. Fatty acids composition of dried fruits of Diospyros blancoi (Db), Landolphia ...

the biosynthetic pathways of saturated and unsaturated fatty acids consist of many steps controlled by various enzymes. one of the methods for improving oil quality is to change the fatty acid profile through genetic manipulation which requires isolation and characterization of the genes and other cis-acting elements, such as the promoter, involved in fatty acid biosynthesis. b-ketoacyl-coa syn...

The study investigated the quantitative and qualitative [chemical] characteristics of fatty acids, carotenoids and tocopherol in some fast foods prepared from a Nigerian eatery. The fatty acid profile was determined by gas chromatography, carotenoids by open column chromatography and visible spectrophotometry, and tocopherol by colorimetric method. The free fatty acid, acid and peroxide values ...