Genetic diversity and identification of fumonisin-producing isolates of Fusarium verticillioides from two provinces in the Philippines were analyzed using molecular techniques. Using a Polymerase Chain Reaction (PCR)-based technique, 49 of the 54 isolates were identified as F. verticillioides, with an amplified product of 800 bp using VERT-1 and VERT-2 primers. Of these, VERTF-1/VERTF-2 primers...

MOTIVATION Oligonucleotide fingerprinting of ribosomal RNA genes (OFRG) is a procedure that sorts rRNA gene (rDNA) clones into taxonomic groups through a series of hybridization experiments. The hybridization signals are classified into three discrete values 0, 1 and N, where 0 and 1, respectively, specify negative and positive hybridization events and N designates an uncertain assignment. This...

This study has been conducted to determine genetic diversity of the common vetch lines and cultivars by using pairwise combinations universal rice primers (URPs). A total number 37 URP marker pairs were tested twenty those provided amplicons in genome. The amplified markers a 83 bands 62 them determined as polymorphic scattered whole average polymorphism rate was calculated 73.5% while informat...

Salvia miltiorrhiza is an economically important floral herb. However, little work has been conducted to further our understanding of the genetics of this herb. In this study, a representative set of germplasm of S. miltiorrhiza populations was used to analyze genetic diversity using amplified fragment length polymorphism (AFLP) methodology. Twenty seven S. miltiorrhiza geographical populations...

Chaetomium spp. are common colonizers of soil and cellulose-containing substrates. Seventeen isolates of Chaetomium spp., which included 15 isolates of C. globosum and one each of C. reflexum and C. perlucidum, were genetically characterized with universal rice primers (URP primers derived from DNA repeat sequences in the rice genome) using polymerase chain reaction (URP-PCR). Out of the 12 URP...

Ten Italian globe artichoke clones belonging to the Romanesco typology were characterized in the western coastal area of Italy (Cerveteri, Rome), using a combination of morphological (UPOV descriptors), biochemical (HPLC analysis), and molecular (AFLP, ISSR, and SSR markers) traits. Significant differences among clones were found for many of the quantitative and qualitative morphological traits...

The sequence of three mitochondrial genes viz., 16S rRNA, Cyt b, COI and one nuclear gene, 28S rRNA of Coranus Curtis species downloaded from the GenBank were subjected to phylogenetic analyses to understand the intrageneric and intraspecific variations and the role of geographical isolation on speciation using CLUSTAL W in MEGA version 5.6. This analysis includes thirteen species of Coranus Cu...

The aim of the study is to determine genetic diversity and relationships among olive cultivars native to Croatia and Turkey. A total of twenty olive (Olea europaea L.) cultivars including fourteen from Croatia and six common cultivars from Turkey were analyzed for genetic diversity and relationships by using six microsatellite markers (DCA05, DCA09, DCA18, GAPU71B, GAPU101, UDO43). The number o...

The aim of this study was the isolation and characterization of thermophilic bacteria from hot springs in Jordan. Ten isolates were characterized by morphological, microscopic, biochemical, molecular, and physiological characteristics. Sequencing of the 16S rDNA of the isolates followed by BLAST search revealed that nine strains could be identified as Bacillus licheniformis and one isolate as T...