considerable amounts of different pollutants, especially crude oils, are present in the persian gulf and its northern region, so biomonitoring the effect of these pollutants on its biota is necessary. ethoxyresorufin o-deethylase (erod), gluthathione s-transferase (gst) and catalase (cat) activity were measured from biochemical level in the gills of mudskipper periophthalmus waltoni collected f...

We evaluated alamarBlue as a metabolic indicator in a standardized assay for the measurement of serum bactericidal activity (SBA) to Haemophilus influenzae type b (Hib) using sera containing natural and vaccine-induced anticapsular (polyribosylribitol phosphate) antibodies. SBA assays with a colorimetric and a fluorometric end point in the presence of alamarBlue were developed and compared to a...

We report a quick, easy and inexpensive fluorometric assay that measures the activity of replication enzymes using PicoGreen. The systems tested include replication of the natural template M13 Gori by E. coli DNA polymerase III holoenzyme and the replication of a synthetic homopolymer by human immunodeficiency virus reverse transcriptase. A direct comparison of the fluorometric assay with the ...

We report a quick, easy and inexpensive fluorometric assay that measures the activity of replication enzymes using Pico-GreenTM. The systems tested include replication of the natural template M13 Gori by E. coli DNA polymerase III holoenzyme and the replication of a synthetic homopolymer by human immunodeficiency virus reverse transcriptase. A direct comparison of the fluorometric assay with th...

background and objectives: numerous procedures in biology and medicine require the counting of cells. direct enumer- ation of colony forming units (cfus) is time-consuming and dreary accurate cell counting on plates with high numbers of cfus is error prone. in this study we report a new indirect cell counting method that was developed based on the use of redsafe fluorometric assay. the usefulne...

Abstract Background Ischemic stroke (IS) is one of the most frequent causes death. Since miRNAs have been illustrated to play an important role in various processes through regulation multiple genes, and platelet function, their utility as novel biomarkers should be determined. Purpose We aimed analyze circulating platelet-derived miR-19a-3p, miR-186-5p, Let-7f, platelet-extracellular vesicles ...

background and objectives: the properties of neuraminidase produced by p. aeruginosa strain pao1 during growth in a defined medium (bhi) was examined and compared with some neuraminidase features of k. pneumoniae in this investigation. materials and methods: the enzyme was isolated from concentrated culture supernatants of p. aeruginosa which was used in a sensitive fluorometric assay by using ...

Oxyresveratrol (OXY) is a small molecule phytochemical which has been reported to have important biological function. The aim of this study was elucidate the gene expression and pathways altered in MCF-7, breast cancer cells following exposure OXY. cytotoxicity different cell lines screened using MTT assay then whole elucidated microarray. selected were also validated by quantitative PCR analys...

BACKGROUND Magnetic resonance spectroscopy studies have suggested above-normal turnover of membrane phospholipids in brains of patients with schizophrenia. One possible explanation for these findings is increased activity of the phospholipid-catabolizing enzyme phospholipase A2 (PLA2). However, attempts to demonstrate higher PLA2 activity in the serum of subjects with schizophrenia have led to ...