the present study reports an efficient protocol for isolation and regeneration of protoplasts from callus of fritillaria imperialis l. there is no published method recommended for protoplast isolation and regeneration from fritillaria imperialis l. a range of factors, which influence the success of isolation and regeneration of f. imperialis protoplasts, were investigated. from the results obta...

in the present study, the effects of milk supplementation on growth and viability of yogurt (streptococcus thermophilus and lactobacillus delbrueckii subsp. bulgaricus) and probiotic bacteria (lactobacillus acidophilus and bifidobacteria) were studied during yogurt production and 33 days of refrigerated storage. the incubation time to reach ph = 4.5 was greatly affected by the addition of milk ...

introduction: infertility is a common phenomenon in modern societies. today, use of assisted reproductive technologies (arts) for treatment of infertility is common, and cryopreservation is one of these technologies. cryopreservation has impact on the function and percentage of fertility of human sperm. in this study, motility, viability, nitric oxide, and dna apoptosis were assessed before and...

this research aimed to investigate the viability of probiotic bacteria ( lactobacillus acidophilus la-5 and bifidobacterium lactis bb-12) and yogurt bacteria ( streptococcus thermophilus and lactobacillus delbrueckii ssp. bulgaricus ) in yogurt during the fermentation, immediately after fermentation and during refrigerated storage (21 d, 4˚c). also the biochemical characteristics of milk as aff...

background and objective: the current study was undertaken to check in vitro different characteristics of lactobacillus plantarum as potential probiotic. these characteristics include viability of probiotic and ph during cold storage, tolerance to acid and bile, and antibiotic resistance. material and methods: samples of yogurt were stored at 4°c and analyzed in time 0, 1, 3, 7, 10 and 14 days ...

the germination power of orchids (orchidaceae family) seems to betoo weak due lack of albumen. the study carried out with various treatments including pollination time and ga3 for breaking dormancy and increasing seed germination of orchids. the effect of pollination time (8 periods from january to august) and gibberellic acid (0, 500, 1000 and 1500 mg l-1) were studied on germination of phalae...

the objective of the present study was to examine the effects of chilling period on semen quality of sannan and jamunapari. semen of goat breeds were collected with artificial vagina and chilled at 5-9˚c. volume, colour and ph were determined on fresh semen. after dilution, microscopic examination was performed for the progressive motility and sperm viability. diluted semen of both breed was co...

in this research, the survival of probiotic microorganisms in yogurts stored at room temperature (cold chain interruption conditions) was studied. milk inoculated with yogurt bacteria (mixed culture of streptococcus thermophilus and lactobacillus delbrueckii ssp. bulgaricus) and a single probiotic culture (l. acidophilus la-5 or bifidobacterium lactis bb-12 or l. rhamnosus hn001 or l. paracasei...

objective(s):bacterial biofilm has been considered responsible for many deaths and high health costs worldwide. their better protection against antibacterial agents compared to free living cells leads to poor treatment efficiency. nanotechnology is promising approach to combat biofilm infections. the aim of the present study was to eradicate staphylococcus epidermidis biofilm with silver nanopa...

Questionnaires were sent to all 1432 general practitioners in the Wessex region to obtain information about their current management of bleeding in early pregnancy. A total of 1290 (90%) returned completed questionnaires. These showed widely varying views about the prognostic importance of particular symptoms and physical signs and about elements of management. Although 96% of the respondents p...