The presence of Vibrio isolates was investigated in cloacal swabs from the Galápagos marine iguana (Amblyrhyncus cristatus). Such unique iguana is endemic to the Galápagos Archipelago, it is listed as vulnerable in the IUCN Red List (2009), and is strictly protected by CITES and Ecuador laws. Our results revealed an uneven isolation frequency of vibrios from animals living in different settings...

AIMS To develop a multiplex PCR targeting the gyrB and pntA genes for Vibrio species differentiation. METHODS AND RESULTS Four pairs of primers targeting gyrB gene of Vibrios at genus level and pntA gene of Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus were designed. This PCR method precisely identified 250 Vibrio species and demonstrated sensitivity in the range of 4 x 10(4) CF...

275 chorea occurs when the function of the corpus striatum is disrupted, the specific abnormality in Sydenham's chorea is unknown. The response to valproic acid suggests that the defect affects the GABA neurotransmitter system. Possibly the difference in effectiveness of valproic acid between Sydenham's chorea and Huntington's chorea reflects the degree of severity of the pathologic process or,...

The ability of Vibrio alginolyticus strains isolated from a bathing and fishing area (Khenis, Centre of Tunisia) to adhere to both biotic and abiotic surfaces was evaluated in the present work. The biochemical, physiological and enzymatic activities of all strains was also investigated. Three morphotypes of V. alginolyticus were obtained on Congo red agar and only 14 strains produced black colo...

NhaB is a bacterial Na(+)/H(+) antiporter with unique topology. The pH dependence of NhaB from Vibrio alginolyticus differs from that of the Escherichia coli NhaB homolog. Replacement of Asp-147 with Glu made high H(+) concentrations a requirement for the NhaB activity. Replacement of Asp-147 with neutral amino acids inactivated NhaB.

To investigate the Na(+)-driven flagellar motor of Vibrio alginolyticus, we attempted to isolate its C-ring structure. FliG but not FliM copurified with the basal bodies. FliM proteins may be easily dissociated from the basal body. We could detect FliG on the MS ring surface of the basal bodies.

The orange-spotted grouper (Epinephelus coioides) is an important marine farmed fish in China. It affected by the bacterial pathogen Vibrio alginolyticus, which causes high mortality and substantial economic losses. We studied transcriptional changes of IgZ gene E. coioides following V. alginolyticus stimulation investigated distribution different tissues. highest expression level occurred head...