background: cholera, an infectious disease caused by vibrio cholerae , is primarily transmitted by ingestion of contaminated food or water. in severe cases, cholera may lead to severe dehydration, metabolic acidosis, and ultimately, hypovolemic shock and death.   methods: in this study v.cholerae non-o1 was cultured in suitable media. lps was extracted from the surface of  bacteria by hot pheno...

background: epidemic and endemic cholera is a major public health problem for many   countries. aim of this study was to evaluate ap-pcr for investigation of clonal relatedness among the strains of vibrio cholerae recovered from an out­break occurred in different parts of iran in 2005. methods: the study was conducted during the cholera outbreak occurred in some of provinces in iran in summer 2...

Vibrio spp. of clinical interest from the Arno River basin (Tuscany, Italy) were investigated in this study. Vibrios were isolated from 70% of water samples. Vibrio cholerae non-O1 was the most prevalent species (82% of isolates), followed by Vibrio mimicus (10%) and Vibrio metschnikovii (8%). Recovery of vibrios was correlated with temperature, pH, and various indicators of municipal pollution...

Vibrio cholerae is a human pathogen that causes mild to severe diarrheal illnesses and has major public health significance. The aim of this study was to investigate the effects of antimicrobial activity of the gold nanoparticles on Vibrio cholera. Gold and Silver nanoparticles are chemically synthesized. Standard strain of Vibrio cholerawas cultured in a nutrient broth. Minimum inhibitory conc...

Vibrio cholerae is a human pathogen that causes mild to severe diarrheal illnesses and has major public health significance. The aim of this study was to investigate the effects of antimicrobial activity of the gold nanoparticles on Vibrio cholera. Gold and Silver nanoparticles are chemically synthesized. Standard strain of Vibrio cholerawas cultured in a nutrient broth. Minimum inhibitory conc...

Cholera outbreak is more common in developing countries. The causative agent of the disease is Vibrio cholerae strains O1 and O139. Traditional diagnostic testing for Vibrio is not always reliable, because Vibrio can enter a viable but non cultivable state. Therefore, nucleic acid-based tests have emerged as a useful alternative to traditional enrichment testing.       In this investigation, a...

The hygienic and pathogenic consequences of the isolation of Vibrio cholerae NAG from the conjunctiva of two ducklings, from brackish water samples in their surroundings, and from the intestinal contents of a duckling, are discussed in relation to present knowledge of the occurrence of Vibrio cholerae NAG in man and animals.

Vibrio cholerae 2012EL-1759 is an environmental isolate from Haiti that was recovered in 2012 during a cholera outbreak. The genomic backbone is similar to that of the prototypical V. cholerae O1 classical biotype strain O395, and it carries the Vibrio pathogenicity islands (VPI-1 and VPI-2) and a cholera toxin (CTX) prephage.

Epithelial cells participate in the innate immune response to pathogenic bacteria by elaborating chemokines. This study examined the effect of Vibrio cholerae and Lactobacillus rhamnosus GG on inflammatory chemokine gene expression in the HT29 human intestinal epithelial cell line. HT29 cells were exposed to V. cholerae 0139, Lactobacillus or both for 2 h and cultured further thereafter for 4 h...

The human pathogen Vibrio cholerae employs several adaptive mechanisms for environmental persistence, including natural transformation and type VI secretion, creating a reservoir for the spread of disease. Here, we report whole-genome sequences of 26 diverse V. cholerae isolates, significantly increasing the sequence diversity of publicly available V. cholerae genomes.