A tissue culture (TC)-fluorescent antibody (FA) technique for the measurement of rabies-neutralizing antibody was found to be reliable and comparable to the standard mouse serum neutralization test. This test was performed with BHK-21 cells infected with the ERA vaccine virus strain on Lab-Tek TC chamber slides. A Flury high egg passage (HEP) rabies virus strain grown on continuous line of Afri...

1. Titration and neutralization of the Western strain of equine encephalomyelitis virus can be carried out in vitro by means of tissue culture. 2. The in vitro titration test as presented is a more sensitive method than animal inoculation. 3. Tissue culture may be better than animal inoculation for the detection of small amounts of virus. 4. The neutralization obtained in tissue culture is 100 ...

The correlation between the modified counterimmunoelectrophoresis test and the indirect fluorescent-antibody test for detecting rabies virus antibodies in human sera was studied, using as a reference the standard mouse neutralization test. Higher sensitivity was observed with the counterimmunoelectrophoresis technique than with the indirect fluorescent-antibody method. The counterimmunoelectrop...

The correlation between the modified counterimmunoelectrophoresis test and the indirect fluorescent-antibody test for detecting rabies virus antibodies in human sera was studied, using as a reference the standard mouse neutralization test. Higher sensitivity was observed with the counterimmunoelectrophoresis technique than with the indirect fluorescent-antibody method. The counterimmunoelectrop...

The time interval between administering the serum and the virus was found to influence the results of the in vivo mouse protection test for foot-and-mouth disease antibodies. In particular, for both IgG and IgM antibodies to strain A12 virus, the mouse protection index increased from zero to a maximum at about 6 h and remained high for at least five days. Variations in the antiserum concentrati...

Various strategies for dealing with COVID-19 have been carried out since the WHO declared as an international health emergency. One of preventive is development vaccines. vaccines developed worldwide. As April 13, 2021, there were 184 vaccine candidates in pre-clinical phase and 16 currently III clinical trials using several platforms, such inactivated viruses, vector protein subunits, mRNA. Cl...

foot and mouth disease (fmd) is a highly contagious viral disease of ruminant which causes fever and postule on mouth, hoof and teat. the main purpose of this study was to determine and charachtrize isolated fmd virus from iran between 2005 - 2006, and to compare it with vaccine virus strains. after preparation of samples, serological test for typing of virus was performed. in order to virus is...

This study aimed to investigate the duration of humoral immune responses to dengue virus (DENV) infection in Japanese who experienced acute febrile illness with hemorrhagic manifestations 70 years ago, when an epidemic of dengue occurred in Nagasaki, Japan, from 1942 to 1944. A Japanese volunteer requested serological diagnosis of DENV infection in 2014 and donated blood sample to measure the a...

To the Editor: Saffold virus (SAFV) is a new human virus belonging to the genus Cardiovirus of the family Picornaviridae (1–6). The virus has also been named human Theiler's-like cardiovirus (4). To date, 8 SAFV genotypes are known, based on molecular variation in the P1 region, which codes for the viral capsid (5). SAFVs are detected in respiratory and fecal samples from infants and children <...

An indirect ELISA incorporating a protein A-peroxidase conjugate was developed for detecting antibodies to swine vesicular disease virus (SVDV) in pig sera. This test and a conventional virus neutralization test were found to be equally sensitive. A total of 2846 pig sera collected from various abattoirs in South Africa were tested using the indirect ELISA. No serological evidence of infection ...