25-OH Vitamin D3 and 25-OH Vitamin D2 were determined by rapid, simple and validated LC/MS/MS method without using SPE and isotopic standard. The relative standard deviations (RSD) were found to be 3.1 and 2.8 % for 25-OH Vitamin D3 and 25-OH Vitamin D2, respectively. The correlation coefficient was grater than 0.99 for both analytes in the calibration range. The recoveries at 100 μg L concentr...

background: the aim of this preliminary study was to assess the safety and efficacy of high-dose oral vitamin d3 supplementation during pregnancy in women with multiple sclerosis (ms) in isfahan, iran. methods: in a single center open-label randomized, controlled clinical phase i/ii pilot study, 15 pregnant women with confirmed ms with low serum 25-hydroxyvitamin d (25(oh)d) levels were randoml...

Colorectal cancer (CRC) is an emerging global problem with the rapid increase in its incidence being associated with an unhealthy lifestyle. Epidemiological studies have shown that decreased levels of vitamin D3 significantly increases the risk of CRC. Furthermore, negative effects of vitamin D3 deficiency can be compensated by appropriate supplementation. Vitamin D3 was shown to inhibit growth...

Emerging evidence from preclinical, clinical and epidemiological studies suggests that vitamin D3 plays vital roles in several diseases in addition to bone disorders. According to new medical evidence, it is being recommended that vitamin D3 intake to be increased for maximal benefits in human health. However, it is necessary to consider potential side effects of increased intake of vitamin D3....

background and aims: 25-hydroxy vitamin d3 is recognized to be an essential element for healthy body; however, its deficiency has been identified as a risk factor for thyroid disease, diabetes mellitus and other autoimmune diseases and infectious diseases. thyroid disease is common in the general population that its prevalence increases proportional to age. subjects with poorly controlled diabe...

The physiological roles played by hepatocytes and nonparenchymal cells of rat liver in the metabolism of vitamin D3 have been investigated. Tritium-labeled vitamin D3 dissolved in ethanol was administered intravenously to two rats. Isolation of the liver cells 30 and 70 min after the injection showed that vitamin D3 had been taken up both by the hepatocytes and by the nonparenchymal liver cells...

Three experiments were conducted to determine whether feeding 25-hydroxyvitamin D3 (25-OH D3) or 1,25-dihydroxyvitamin D3 (1,25-(OH)2 D3) improves the tenderness of longissimus dorsi (LD), semimembranosus (SM), and infraspinatus (IF) muscles similar to supplemental vitamin D3 without leaving residual vitamin D3 and its metabolites in muscle. In the first two experiments, 24 crossbred steers wer...

The formation of fatty acid esters of vitamin D3 was demonstrated in rat skin exposed to artificial ultraviolet rays by using multi-dimensional high-performance liquid chromatography, ultraviolet spectrophotometry, and gas-liquid chromatography-mass spectrometry. This result indicated that the fatty acid esters of 7-dehydrocholesterol in rat skin (at least 80% of 7-dehydrocholesterol in rat ski...

Background: Inflammatory responses in individuals exposed to sulfur mustard occur in some organs such as lung, skin and eyes. These organs manifest overreaction in cellular and humoral immune responses. Over a long period, the immune responses often continues toward chronic inflammatory processes with some interventions. Obviously some elements have been deregulated. Therefore, this study evalu...