An accelerated water-loss was observed in decapitated individuals, no equivalent increase being obtained following severance of the nervous connectives in the neck. Injection of brain and, to a lesser extent, corpus cardiacum extract resulted in a significant reduction in the rate of loss of water from decapitated individuals. The accelerated water-loss observed following decapitation appeared ...

Proteins that bind intracellularly are surrounded by other macromolecules. Macromolecular crowding has been shown to impact protein folding and binding, but its effects on the electrostatics of protein binding have not been thoroughly studied. Two ways crowding can affect binding are via loss of water mobility and water depletion. Crowding causes loss of water mobility because more water molecu...