Heavy nitrogen (N) application to gain higher yield of wheat (Triticum aestivum L.) resulted in increased production cost and environment pollution. How to diminish the N supply without losing yield and/or quality remains a challenge. To meet the challenge, we integrated and expressed a tobacco nitrate reductase gene (NR) in transgenic wheat. The 35S-NR gene was transferred into two winter cult...

Residue from cultivars of spring wheat (Triticum aestivum L.), winter wheat, and spring barley (Hordeum vulgare L.) was characterized for fiber and nutrient traits using reference methods and near-infrared spectroscopy (NIRS). Calibration models were developed for neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), carbon (C), sulfur (S), nitrogen (N), and C:...

Polymerase chain reaction (PCR) with degenerate primers was utilized for partial cloning of the MADS box gene family from wheat (Triticum aestivum L.). PCR products corresponding to a part of the MADS box region were cloned and sequenced. Eleven individual clones sequenced were classified into seven types on the basis of the nucleotide sequence and five types on the deduced amino acid sequence,...

Virus-induced gene silencing (VIGS) is a powerful technique to study gene function in plants. However, very few VIGS vectors are available for monocot plants. Here we report that Foxtail mosaic virus (FoMV) can be engineered as an effective VIGS system to induce efficient silencing of endogenous genes in monocot plants including barley (Hordeum vulgare L.), wheat (Triticum aestivum) and foxtail...

Rhopalosiphum padi L. causes significant damage to winter wheat (Triticum aestivum L.), even without obvious aboveground symptoms of injury. Our objectives were to develop a juvenile-plant bioassay for bird cherry-oat aphid (BCOA) resistance that allows rapid phenotypic differentiation. Central features of the bioassay include root and shoot weight measurements of 3-week-old seedlings produced ...

AN ECOLOGICALLY RELEVANT SOIL EXTRACTION PROCEDURE SEPARATED TWO TYPES OF MOLECULES IMPORTANT FOR BACTERIA: flavonoids and small hydrophilic organic compounds. Two flavonoids, identified previously as inducers of nodulation genes in Rhizobium meliloti, were detected in rhizosphere soil from alfalfa (Medicago sativa L.). In addition, biologically significant quantities (micromoles per kilogram) ...

Currently, food security depends on the increased production of cereals such as wheat (Triticum aestivum L.), which is an important source of calories and protein for humans. However, cells of the crop have suffered from the accumulation of reactive oxygen species (ROS), which can cause severe oxidative damage to the plants, due to environmental stresses. ROS are toxic molecules found in variou...

This study deals with hybrids between Aegilops geniculata and bread wheat, Triticum aestivum L., detected in two Ae. geniculata populations in the "Meseta Central", Spain's central plateau where wheat is a major crop. Morphological traits and pentaploid chromosome numbers were used to identify hybrids in 2004 and 2005. The frequency of hybridization under natural conditions was calculated for o...

An imidazolinone-tolerant wheat (Triticum aestivum L. em Thell) mutant in the winter wheat cultivar Fidel has been identified and characterized. The mutant was isolated from a population derived through seed mutagenesis of the variety with an aqueous solution containing sodium azide. Imidazolinone-tolerant wheat seedlings were selected from the M(2) generation of the population in the presence ...

The bread wheat (Triticum aestivum L.) genotype "Chinese Spring" ("CS") is the reference base in wheat genetics and genomics. Pericentric rearrangements in this genotype were systematically assessed by analyzing homoeoloci for a set of nonredundant genes from Brachypodium distachyon, Triticum urartu, and Aegilops tauschii in the CS chromosome shotgun sequence obtained from individual chromosome...