Based on the zymography analysis, Bacillus sp. DJ-4 (screened from Doen-Jang, a Korean traditional fermented food) secretes seven extracellular fibrinolytic enzymes (EFEs; 68, 64, 55, 45, 33, 27, and 13 kDa) in culture broth. These seven EFEs were analyzed by newly applied SDSfibrin zymography combined with gradient polyacrylamide (SDS-FZGP). This improved gel system was used with a 5-20% acryl...

objective(s):n-myc downstream regulated gene 2 (ndrg2) is a candidate gene for tumor suppression. the expression of ndrg2 is down-regulated in several tumors including lung cancer. the aim of this study was to explore the effect of ndrg2 overexpression on invasion, migration, and enzymatic activity of matrix metalloproteinase-2 (mmp-2) and -9 (mmp-9) in human lung adenocarcinoma a549 cells.  ma...

BACKGROUND Various molecules participate in implantation and maintaining endometrial function during gestation. The remodeling of endometrial matrices is a necessary process in the coordination of gestational progress. Matrix-metalloproteinases (MMPs) like gelatinases (MMP-2 and -9) and collagenase (MMP-1) are considered to play important roles in this process. We examined MMP-2 and -9 expressi...

Microplastics provide a new ecological niche for microorganisms, and the accumulation levels of microplastics (MPs) in terrestrial ecosystems are higher than those marine ecosystems. Here, we applied zymography to investigate how MPs — polyethylene [PE], polyvinyl chloride [PVC]) at two (0.01% 1% soil weight) impacted spatial distribution hydrolases, nutrient availability, rice growth paddy soi...

Antheraea assamensis Helfer is an endemic non-mulberry lepidopteran species of North East India with high commercial demand for its golden hued silk. suffers from a protozoan disease pebrine which lead to death the silkworm. In this study, effect various factors (starvation, feeding, temperature, pH, Ca ion and larval stage) in regulation amylase secretion compared healthy diseased muga Moreove...