Background: Coastal areas have a high bacterial diversity, which can be used as source of pharmaceutical substances, including the fibrinolytic enzyme. Fibrinolytic enzymes lyse blood clots by degrading fibrin. These are important for treating circulatory disorders, e.g., pulmonary embolism, deep vein thrombosis, and myocardial infarction. Objective: This study aimed to determine activity crude...

1. Abstract 2. Introduction 3. Materials and methods 3.1. Exogenous PLA2s 3.2. ExPLI 3.3. Cell culture 3.4. Determination of basement membrane invasiveness 3.5. Determination of cell chemotaxis 3.6. Determination of MMP activity (Zymography) 3.7. Determination of cell PLA2 activity 3.8. Determination of exogenous PLA2 activity 3.9. Identification of cell sPLA2 and sPLA2 receptor expression 3.10...

Stroke is one of the leading causes death and disability worldwide. However, treatment options for ischemic stroke remain limited. Matrix-metalloproteinases (MMPs) contribute to brain damage during strokes by disrupting blood-brain barrier (BBB) causing edemas. Carnosine, an endogenous dipeptide, was found us others be protective against injury. In this study, we investigated whether carnosine ...

Proteases are being widely used in various industries like detergent, leather, food and pharmaceuticals.Protease was purified to homogeneity from the seeds of Artocarpus heterophyllus. The enzyme found be a tetramer having molecular mass 74 kDa. Gelatin zymography showed clear band proteolysis. isolated serine protease, as indicated by its inhibition with PMSF. stable at broad pH temperature ra...

Introduction and Aim: It has been proposed that µ-calpain is responsible for neuronal survival, while m-calpain – the degeneration. can be assumed "susceptibility" to damage factor neurons in different CNS regions depends on content/activity of calpain isoforms. We analyzed mRNA levels activity µ-and structures rats. Materials Methods: After decapitation intact male Wistar rats prefrontal corte...

Background: Proteolytic enzymes have an important role in variety of physiological and pathological functions. They have been used in therapeutic and pharmaceutical applications. Characterizations of extracellular proteases from various strains of S. marcescens indicate that most strains produce a very similar major metalloprotease. This metalloprotease (serrapeptidase, serrapeptase) is an impo...

We studied the activity of matrix metalloproteinases (MMP) 2 and 9 generated by cultured rabbit corneal epithelium cells that had been stimulated with tumor necrosis factor alpha (TNF-α), to investigate the possible regulative mechanisms of MMP-2/9 and their potential effect on corneal inflammatory diseases. The rabbit corneal epithelium cells were cultured in vitro and incubated with different...