objective(s) the aim of the present study was to compare the therapeutic indices of several agents used in treatment of inflammatory conditions which included: vitamin e, hydro-alcoholic extracts of glycyrrhiza glabra, matricaria aurea, dexamethasone, piroxicam and diclofenac using wehi-164 fibrosarcoma cells. materials and methods cytotoxicity evaluation was based on vital dye exclusion assay....

The balance between matrix metalloproteinases (MMPs) and their inhibitors, the tissue inhibitors of metalloproteinases (TIMPs), is largely responsible for the remodeling of tissues. Deregulation of this balance is a characteristic of extensive tissue degradation in certain degenerative diseases. To analyze the role of MMPs and TIMPs in tissue remodeling under normal and pathological conditions,...

Gingipains (RgpA, RgpB, and Kgp) are major virulence factors of the periodontitis-causing bacterium Porphyromonas gingivalis. Isolation gingipains from crude protein sample P. gingivalis is critical for studying underlying invasion mechanism that contributes to periodontitis, Alzheimer’s disease, cardiovascular disease (CVD). Chromatographic processes molecular cloning two standard techniques o...

In this study, Aspergillus niger lipase after extraction of medium culture was precipitated with different percentages of acetone and purified by ion exchange chromatography using SP-sepharose HP and Q-sepharose HP. The process of purification of the anzyme was studied by electrophoresis and the molecular weight was detected and determined by Zymography using overlying containing phenol red and...

Snake venom contains various bioactive proteins and peptides, of which enzymes make up a significant portion. Desert Black Cobra (Walterinnesia morgani) is venomous snake distributed mainly in the Middle East including southeastern Türkiye. The aim present study to investigate key enzyme activities protein profile W. morgani originating from Sanliurfa province. After determination content, samp...

OBJECTIVE In a trial to improve the diagnostic efficacy of conventional urine cytology we determine survivin RNA and matrix metalloproteinase 2 and 9 in urine of bladder cancer cases. METHOD Voided urine specimens were collected from patients with histologically confirmed bladder urothelial carcinoma (Group 1; n=46), urological patients without urothelial carcinoma (Group 2; n=20), and health...

We have developed a two-dimensional (2D-) gel system of zymography and reverse zymography for the detection and characterization of proteases and protease inhibitors. Isoelectric focusing (IEF) agarose gels with pH gradients were employed for separation in the first-dimension and sodium dodecyl sulfate (SDS)-polyacrylamide gel copolymerized with gelatin used for the second dimension. Proteases ...