Binding mode was demonstrated by Brown and Lantz. They demonstrated that enzymes bind to a fixed substrate in SDS-polyacrylamide gels and they do not migrate into the separating gel and hydrolyze the substrate. We found two types of binding modes. One was formed because of binding between the enzyme and substrate, and the other was due to a high pI value (8.8) of the enzyme. These two types wer...

References 1. Tanumihardjo SA, Cheng JC, Permaesih D, Muherdiyantiningsih, Rustan E, Muhilal, et al. Refinement of the modified-relative-dose-response test as a method for assessing vitamin A status in a field setting: experience with Indonesian children. Am J Clin Nutr 1996;64: 966–71. 2. de Pee S, Yuniar Y, West CE, Muhilal. Evaluation of biochemical indicators of vitamin A status in breast-f...

Cathepsin K, the most potent mammalian collagenase, has been implicated in osteoporosis, cancer metastasis, atherosclerosis, and arthritis. Although procathepsin K is stable and readily detected, the active mature cathepsin K eludes detection by in vitro methods due to its shorter half-life and inactivation at neutral pH. We describe, for the first time, reliable detection, visualization, and q...